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Method for establishing HPLC fingerprint spectrum of Lysimachia capillipes Hemsl. medicinal material and fingerprint spectrum thereof

A technology of fingerprint and stalk vanilla, applied in material separation, material analysis, measuring device, etc., can solve the problems of less stalk vanilla, weak and other issues

Active Publication Date: 2019-10-01
江西省药品检验检测研究院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, there are few researches on the quality control of herb and mainly focus on the content determination of a single index component, which is very weak for the quality control of herb.

Method used

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  • Method for establishing HPLC fingerprint spectrum of Lysimachia capillipes Hemsl. medicinal material and fingerprint spectrum thereof
  • Method for establishing HPLC fingerprint spectrum of Lysimachia capillipes Hemsl. medicinal material and fingerprint spectrum thereof
  • Method for establishing HPLC fingerprint spectrum of Lysimachia capillipes Hemsl. medicinal material and fingerprint spectrum thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0105] Chromatographic conditions and system suitability test using octadecylsilane bonded silica gel as filler (column length 25cm, inner diameter 4.6 mm, particle size 5μm); acetonitrile as mobile phase A, 0.3% phosphoric acid solution as mobile phase B. Carry out gradient elution as specified in the table below; the detection wavelength is 254nm; the column temperature is 30°C; the flow rate is 1.0ml per minute.

[0106]

[0107] Preparation of reference solution

[0108] Precise measurement of fine-stemmed vanilla reference substances quercetin-3-O-(2”, 6”-di-O-α-rhamnopyranosyl)-β-galactoside, kaempferol-3-O- [2-Glucopyranosyl (1→3)-rhamnopyranose-6-rhamnopyranose]-β-D-galactopyranoside, kaempferol-3-O-{[β-D -Glucopyranosyl(1→3)]-4-O-(E-p-coumarin)}-α-L-rhamnopyranose-(1→6)-β-D-glucopyranoside-7 -O-(4-O-acetyl)-α-L-rhamnopyranoside, kaempferol-3-O-{[β-D-xylopyranosyl (1→3)-α-L- Rhamnopyranose (1→6)][α-L-rhamnopyranose-(1→2)]}-β-D-3-trans-p-coumaroyl glucopyranoside ...

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Abstract

The invention discloses a method for establishing an HPLC fingerprint spectrum of a Lysimachia capillipes Hemsl. medicinal material and a fingerprint spectrum thereof. The method comprises the following steps of (1) preparing a reference substance solution; (2) preparing a test solution: (3) preparing the fingerprint spectrum; and (4) carrying out similarity evaluation. The method disclosed by theinvention is simple, rapid, accurate and good in repeatability, and provides a powerful guarantee for comprehensively and effectively controlling the quality of the Lysimachia capillipes Hemsl medicinal material.

Description

technical field [0001] The invention relates to the technical field of fingerprints of traditional Chinese medicinal materials, in particular to a method for establishing fingerprints of fine-stemmed herbs and fingerprints thereof. Background technique [0002] Traditional Chinese medicine fingerprint refers to the spectrum that can mark the chemical characteristics of certain Chinese medicinal materials or traditional Chinese medicine preparations after proper processing and certain analysis methods. It can more comprehensively reflect the types and quantities of chemical components contained in medicinal materials, and effectively reflect the integrity and comprehensive effects of traditional Chinese medicine ingredients. It has been widely used in the analysis, identification and quality control of traditional Chinese medicine due to its fast and accurate characteristics. One of the most effective technical means at this stage has also been recognized by the international...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/06
CPCG01N30/02G01N30/06G01N2030/047
Inventor 杨毅生洪挺钟瑞建章光文王栋钱媛张昆艳
Owner 江西省药品检验检测研究院
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