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Kit for diagnosing bladder cancer

A bladder cancer and kit technology, applied in the field of biomedicine, can solve the problems of lack of early diagnosis markers for bladder cancer and patient suffering

Active Publication Date: 2019-10-11
THE SECOND HOSPITAL OF TIANJIN MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is a lack of early diagnostic markers for bladder cancer clinically, and cystoscopy will bring pain to patients

Method used

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  • Kit for diagnosing bladder cancer
  • Kit for diagnosing bladder cancer
  • Kit for diagnosing bladder cancer

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] A diagnostic kit for bladder cancer, comprising: BUB1 antibody, xylene, ethanol, 3% H 2 o 2 solution, 1% BSA blocking solution, DAB chromogenic reagent, hematoxylin, horseradish peroxidase-labeled goat anti-mouse IgG, 0.01M pH=6.0 citrate buffer and 0.01M PBS.

Embodiment 2

[0022] 3×10 3 5637 and T24 bladder cancer cells knocked down BUB1 protein and control cells (normal 5637 and T24 cells) were seeded in 96-well plates, and treated with MTT method (3-(4,5-dimethyl -2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide) was used to detect the growth activity of tumor cells, and the absorbance of the cells was detected by a tube photometer, and the average value and standard deviation were calculated. The results showed that compared with the control cells, the growth ability of bladder cancer cells knocked down BUB1 was significantly weakened in vitro ( figure 1 A is T24 cells, B is 5637 cells), suggesting that BUB1 may increase the growth and viability of bladder cancer cells.

Embodiment 3

[0024] 2×10 4 The 5637 bladder cancer cells knocked down BUB1 protein and the control cells (normal 5637 cells) were inoculated on the upper layer of the polycarbonate membrane chamber (Corning company product), and after 24 hours of culture, the cells passing through the polycarbonate membrane were stained with crystal violet Afterwards, observe and take pictures under a microscope, count the cancer cells passing through the polycarbonate membrane in each field of view, calculate the average value and calculate the standard deviation. The results showed that compared with the control cells, the migration ability of bladder cancer cells knocked down BUB1 protein was significantly reduced in vitro ( figure 2 A is T24 cells, B is 5637 cells), suggesting that BUB1 protein may increase the metastasis of bladder cancer cells.

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Abstract

The invention discloses a kit for diagnosing bladder cancer, and the kit comprises a BUB1 antibody, xylene, ethanol, 3% of H2O2 solution, 1% of BSA blocking solution, a DAB chromogenic reagent, hematoxylin, horseradish peroxidase labeled goat anti-mouse IgG, 0.01M of citrate buffer of which pH=6.0 and 0.01M of PBS. The kit for diagnosing bladder cancer is based on the strong correlation between BUB1 and bladder cancer in clinical data and basic biological experimental data of a preliminary study. Based on the correlation between the expression level of the BUB1 protein and the bladder cancer,the expression level of the protein can be detected by taking the protein as a molecular marker to help diagnose the bladder cancer.

Description

technical field [0001] The invention belongs to the technical field of biomedicine and relates to a kit for diagnosing bladder cancer. Background technique [0002] Urothelial carcinoma of the bladder is the most common malignant tumor of the urinary system worldwide, with approximately 76,960 new cases and 16,390 deaths each year in the United States. According to the latest statistical data published in the Journal of Clinical Oncology in 2017, the incidence of bladder cancer has ranked the fourth most common malignant tumor in American men [1] . In my country, its morbidity and mortality rate rank first in genitourinary system tumors. In 2015, more than 80,000 patients were newly diagnosed with bladder cancer nationwide, resulting in more than 30,000 deaths. More than 70% of the tumors were superficial cancers at the first diagnosis, and 30% of the tumors were invasive bladder cancers. [2,3,4] . Additionally, approximately 60% of superficial bladder cancers recur and ...

Claims

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Application Information

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IPC IPC(8): G01N33/68G01N33/577G01N33/574
CPCG01N33/6893G01N33/577G01N33/57407
Inventor 蒋宁
Owner THE SECOND HOSPITAL OF TIANJIN MEDICAL UNIV
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