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Emasculation hybridization method for sorghum

A hybridization method and technology for sorghum, applied in the fields of botanical equipment and methods, plant genetic improvement, animal repellent, etc., can solve the problems of low success rate of sorghum emasculation and low seed setting rate, and achieve the improvement of emasculation rate and emasculation. The effect of increasing the success rate and avoiding pollution

Active Publication Date: 2019-10-15
RICE & SORGHUM INST SICHUAN ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Aiming at the above-mentioned prior art, the present invention provides a sorghum emasculation hybridization method to solve the problems of sorghum emasculation success rate and low seed setting rate

Method used

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  • Emasculation hybridization method for sorghum

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] A sorghum emasculation hybridization method includes the following steps:

[0021] S1: On the day before emasculation, select sorghum plants that are growing well and entering the flowering period, remove the flowering spikelets and late-developing branches from the female sorghum ears, and leave the branches that are about to bloom in the middle and upper parts, and comb them For spikelets that are too dense in the middle and upper part, complete one whole ear processing of sorghum;

[0022] S2: Put the female sorghum ears from the whole ear into a transparent plastic bag containing detasseling liquid, and the tip of the ear is about 5cm away from the level of the detasseling liquid in the bag, and the plastic bag is fastened tightly to the ear On the rod, overnight; the castration solution includes the following components in a concentration ratio: ethephon 0.1g / L, gibberellin 0.25mg / L, tribenuron 0.01mg / L, and the solvent of the castration solution is acetone and water. ...

Embodiment 2

[0026] A sorghum emasculation hybridization method includes the following steps:

[0027] S1: On the day before emasculation, select sorghum plants that are growing well and entering the flowering period, remove the flowering spikelets and late-developing branches from the female sorghum ears, and leave the branches that are about to bloom in the middle and upper parts, and comb them For spikelets that are too dense in the middle and upper part, complete one whole ear processing of sorghum;

[0028] S2: Put the sorghum female parent ears after a whole ear into a transparent plastic bag containing detasseling liquid. The distance between the tip of the ear and the level of the detasseling liquid in the bag is about 4cm, and the plastic bag is fastened tightly on the ear. On the rod, overnight; the castration solution includes the following components in a concentration ratio: ethephon 0.05g / L, gibberellin 0.3mg / L, tribenuron 0.005mg / L, the solvent of castration solution is acetone a...

Embodiment 3

[0032] A sorghum emasculation hybridization method includes the following steps:

[0033] S1: On the day before emasculation, select sorghum plants that are growing well and entering the flowering period, remove the flowering spikelets and late-developing branches from the female sorghum ears, and leave the branches that are about to bloom in the middle and upper parts, and comb them For spikelets that are too dense in the middle and upper part, complete one whole ear processing of sorghum;

[0034] S2: Put the female sorghum ears from the whole ear into a transparent plastic bag containing detasseling liquid, and the distance between the tip of the ear and the level of the detasseling liquid in the bag shall be about 6cm, and the plastic bag should be fastened tightly on the ear On the rod, overnight; the castration solution includes the following components in a concentration ratio: ethephon 0.15 / L, gibberellin 0.2mg / L, tribenuron 0.015mg / L, the solvent of the castration solution...

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Abstract

The invention discloses an emasculation hybridization method for sorghum. The method comprises steps as follows: firstly, a sorghum female ear is subjected to ear shaping treatment, the sorghum femaleear after ear shaping treatment is put in a transparent plastic bag containing an emasculation liquid overnight, the emasculation liquid contains ethephon, gibberellin and tribenuron-methyl, and a solvent of the emasculation liquid is a mixed solution formed by acetone and water in a volume ratio of 1:(8-10) by mixing; then the emasculation liquid in the bag is poured on the anther in the next morning, meanwhile, the rachis is tapped until the anther without pollen scattering drops off completely, and artificial emasculation is completed; finally, the sorghum female ear after artificial emasculation is subjected to secondary ear treatment and then is bagged for isolation; after dew is dried, target male parent pollen is taken for pollinating the female parent, and sorghum hybridization isfinished. With the adoption of the method, the emasculation rate and the setting percentage of the sorghum can be greatly increased, the method is an efficient emasculation hybridization mode, and one new thought is provided for sorghum breeding.

Description

Technical field [0001] The invention belongs to the technical field of plant breeding, and specifically relates to a sorghum emasculation hybridization method. Background technique [0002] The creation of good parental materials is the prerequisite for cultivating strong dominant varieties, and sexual hybridization is one of the important means to create high-yield, high-quality, and multi-resistant materials, and then to cultivate excellent new varieties. Artificial emasculation is one of the most important and difficult tasks in sorghum breeding. [0003] The previous sorghum warm soup emasculation method and vibration emasculation method are greatly affected by the environment, such as low efficiency, difficult operation, poor seed setting rate, low success rate, and few seeds obtained. For example, the operation of emasculation with warm soup is complicated, and special devices are needed, and the stalks are often bent and broken due to the tall stalks. Vibration emasculation...

Claims

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Application Information

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IPC IPC(8): A01H1/02A01N27/00A01N57/20A01N43/12A01N45/00A01N47/36A01P21/00
CPCA01H1/02A01N27/00A01N57/20A01N43/12A01N45/00A01N47/36
Inventor 倪先林丁国祥赵甘霖龙文靖汪小楷刘天朋向箭宇李元
Owner RICE & SORGHUM INST SICHUAN ACAD OF AGRI SCI
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