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Application of Kealiinine alkaloids in prevention and treatment of plant virus and bacterial diseases

A plant virus and alkaloid technology, applied in the fields of chemicals, applications, biocides for biological control, etc., can solve the problem that the anti-plant virus and pathogen activity has not been systematically studied and reported.

Active Publication Date: 2019-11-22
NANKAI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0014] So far, although Kealiinine alkaloids have relatively mature synthetic routes and have been found to have certain anticancer activity and artemia killing activity, their other biological activities, especially the activities against plant viruses and pathogens, are still unknown. Not systematically studied and reported

Method used

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  • Application of Kealiinine alkaloids in prevention and treatment of plant virus and bacterial diseases
  • Application of Kealiinine alkaloids in prevention and treatment of plant virus and bacterial diseases
  • Application of Kealiinine alkaloids in prevention and treatment of plant virus and bacterial diseases

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Embodiment 1: Experimental data of Kealiinine alkaloids Ia~Ic

[0022] Ia: gray-green solid, melting point: 277-278°C. 1 H NMR (400MHz, DMSO-d 6 )δ8.88(s, 1H), 7.35(d, J=8.0Hz, 2H), 7.34(s, 1H), 7.25(s, 1H), 7.05(d, J=8.0Hz, 2H), 7.04( s, 1H), 6.70 (s, 2H), 3.86 (s, 3H), 3.83 (s, 3H), 3.54 (s, 3H). 13 C NMR (100MHz, DMSO-d 6 )δ158.4, 157.4, 147.1, 145.1, 134.8, 132.6, 130.6, 124.4, 124.2, 120.3, 113.8, 107.6, 107.1, 101.6, 55.8, 55.6, 28.9.

[0023] Ib: Brown-yellow solid, melting point: 278-280°C. 1 H NMR (400MHz, DMSO-d 6 )δ7.43(d, J=8.0Hz, 2H), 7.38(s, 1H), 7.29(s, 1H), 7.11(s, 1H), 7.05(d, J=8.0Hz, 2H), 6.69( s, 2H), 3.86(s, 3H), 3.83(s, 3H), 3.63(s, 3H), 3.55(s, 3H). 13 C NMR (100MHz, DMSO-d 6 )6157.8, 157.2, 146.9, 146.8, 140.4, 135.0, 132.1, 129.8, 124.0, 123.0, 120.6, 113.3, 106.7, 104.0, 101.1, 55.2, 55.0, 55.0, 28.4.

[0024] Ic: Brown-yellow solid, melting point: 302-304°C. 1 H NMR (400MHz, DMSO-d 6 )δ7.40(s, 1H), 7.17(d, J=8.8Hz, 2H), 7.15(s, 1H),...

Embodiment 2

[0025] Embodiment 2: the mensuration of anti-tobacco mosaic virus activity, assay procedure is as follows

[0026] 1. Virus purification and concentration determination:

[0027] Virus purification and concentration determination were carried out in accordance with the SOP specification for tobacco mosaic virus compiled by the Bioassay Laboratory of the Institute of Elements, Nankai University. After the crude virus extract was centrifuged twice with polyethylene glycol, the concentration was measured and refrigerated at 4°C for later use.

[0028] 2. Compound solution preparation:

[0029] After weighing, the original drug was dissolved in DMF to prepare 1×10 5 μg / mL mother solution, and then diluted with 1‰ Tween 80 aqueous solution to the required concentration; Ningnanmycin preparation was directly diluted with water.

[0030] 3. In vivo protection:

[0031] Select Shanxi tobacco with uniform growth at the 3-5 leaf stage, spray the whole plant, and repeat each treatmen...

Embodiment 3

[0040] Embodiment 3: antibacterial activity test, assay procedure is as follows:

[0041] In vitro bactericidal test, bacterial growth rate determination method (plate method):

[0042] Dissolve a certain amount of medicine in an appropriate amount of acetone, then dilute it with an aqueous solution containing 200ug / mL emulsifier to the required concentration, then draw 1mL of the medicine solution into the petri dish, add 9mL of medium, shake well and make 50ug / mL mL of the drug-containing plate, and a plate with 1 mL of sterilized water added as a blank control. Use a puncher with a diameter of 4mm to cut out the bacterial plate along the outer edge of the hyphae, and move it to the drug-containing plate. Each treatment was repeated three times. Place the culture dish in a constant temperature incubator at 24±1°C. After 48 hours, investigate the expanded diameters of the bacteria discs of each treatment, calculate the average value, and compare with the blank control to c...

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Abstract

The invention relates to application of Kealiinine alkaloids Ia-Ic in prevention and treatment of plant virus and bacterial diseases. The Kealiinine alkaloids Ia to Ic disclosed by the invention showgood plant virus resisting activity, and in particular, the alkaloid Ib shows relatively good tobacco mosaic virus (TMV) resisting activity. Meanwhile, the Kealiinine alkaloids Ia to Ic also show a certain activity of killing plant pathogenic bacteria.

Description

technical field [0001] The invention relates to the application of Kealiinine alkaloids in the prevention and treatment of plant virus pathogens, and belongs to the technical field of agricultural protection. Background technique [0002] The ocean is the largest treasure house of resources on the earth. Its unique marine environment (high salinity, high pressure, anoxic, low (constant) temperature, low (no) light) produces marine natural products with diverse biological activities and rich structures. So far, more than 25,000 natural products of marine biological origin have been discovered, and this number is still increasing by an average of 1,000 species per year (Nat.Prod.Rep.2014, 31(2):160-258.; Nat. Prod. Rep. 2017, 34(3): 235-294.). In the field of medicine, many marine natural products exhibit excellent medicinal activities such as antitumor, antibacterial, antiviral, and anticardiovascular diseases (Journal of Chengde Medical College, 2017, 34(4): 332-336.); In ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N43/52A01P1/00A01P3/00
CPCA01N43/52
Inventor 汪清民李刚刘玉秀王兹稳
Owner NANKAI UNIV
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