Application of alkaloid arundine and derivatives thereof in prevention and control of plant viruses and pathogenic bacteria
A technology of plant viruses and derivatives, applied in the direction of chemicals, applications, biocides, etc. for biological control
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Embodiment 1
[0014] Example 1: Synthesis of Compounds I-1~I-25: 0°C, under stirring, add I 2 (0.5 mmol). Keep stirring at this temperature for 30min, add 5% Na 2 S 2 o 3 solution (20 mL) to quench the reaction. The reaction solution was extracted with ethyl acetate (3×50mL), the organic phase was washed with saturated brine (100mL), anhydrous Na 2 SO 4 Dry, filter and concentrate. The residue was subjected to column chromatography with petroleum ether and ethyl acetate (5:1, v / v) to obtain compounds I-1~I-25.
[0015] Bis(1H-indol-3-yl)methane (I-1, arundine). Pink solid; yield 61%; melting point 161-163°C; 1 H NMR (400MHz, DMSO-d 6 )δ10.72(s, 2H), 7.52(d, J=7.9Hz, 2H), 7.31(d, J=8.1Hz, 2H), 7.13(s, 2H), 7.05-6.99(m, 2H), 6.94-6.88(m, 2H), 4.12(s, 2H); 13 C NMR (100MHz, DMSO-d 6 )δ 136.4, 127.2, 122.7, 120.7, 118.6, 118.0, 114.2, 111.3, 20.9.C 17 h 15 N 2 [M+H] + 247.1230, found (ESI + )247.1235.
[0016] Bis(5-bromo-1H-indol-3-yl)methane (I-2). Pink solid; yield 53%; melt...
Embodiment 2
[0040] Embodiment 2: the assay of anti-tobacco mosaic virus activity, assay procedure is as follows:
[0041] 1. Virus purification and concentration determination:
[0042] Virus purification and concentration determination were carried out in accordance with the SOP specification for tobacco mosaic virus compiled by the Bioassay Laboratory of the Institute of Elements, Nankai University. After the crude virus extract was centrifuged twice with polyethylene glycol, the concentration was measured and refrigerated at 4°C for later use.
[0043] 2. Compound solution preparation:
[0044] After weighing, the original drug was dissolved in DMF to prepare 1×10 5 μg / mL mother solution, and then diluted with 1‰ Tween 80 aqueous solution to the required concentration; Ningnanmycin preparation was directly diluted with water.
[0045] 3. In vivo protection:
[0046] Select Shanxi tobacco with uniform growth at the 3-5 leaf stage, spray the whole plant, and repeat each treatment 3 t...
Embodiment 3
[0057] Embodiment 3: antibacterial activity test, assay procedure is as follows:
[0058] In vitro bactericidal test, bacterial growth rate determination method (plate method):
[0059] Dissolve a certain amount of medicine in an appropriate amount of acetone, then dilute it with an aqueous solution containing 200 μg / mL emulsifier to the required concentration, then draw 1 mL of the medicine solution into the petri dish, then add 9 mL of medium, shake well and make 50 μg / mL mL of the drug-containing plate, and a plate with 1 mL of sterilized water added as a blank control. Use a puncher with a diameter of 4mm to cut out the bacterial plate along the outer edge of the hyphae, and move it to the drug-containing plate. Each treatment was repeated three times. Place the culture dish in a constant temperature incubator at 24±1°C. After 48 hours, investigate the expanded diameters of the bacteria discs of each treatment, calculate the average value, and compare with the blank con...
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