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Preparation method of amino acid solution, amino acid solution, amino acid water-soluble fertilizer and application of amino acid water-soluble fertilizer

An amino acid and water-soluble fertilizer technology, applied in the field of preparation of amino acid water-soluble fertilizer, amino acid liquid, and amino acid liquid, can solve the problems of irrational use of chemical fertilizers, soil compaction, soil fertility decline, etc., to promote photosynthesis, increase crop yield, improve effect of growth

Active Publication Date: 2019-12-06
FUJIAN SANJU BIOLOGICAL SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0002] Due to people's continuous planting, long-term unreasonable use of chemical fertilizers, and repeated application of large amounts of water-soluble fertilizers, the soil is severely compacted, soil acidified, and soil salinized, resulting in an increasingly serious decline in soil fertility. The occurrence of soil-borne diseases eventually leads to the decline of crop quality
[0003] Resources such as soybean meal are rich in amino acids, and the sources are extensive and the cost is low, but to a certain extent, they have not yet reached high-value utilization

Method used

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  • Preparation method of amino acid solution, amino acid solution, amino acid water-soluble fertilizer and application of amino acid water-soluble fertilizer
  • Preparation method of amino acid solution, amino acid solution, amino acid water-soluble fertilizer and application of amino acid water-soluble fertilizer
  • Preparation method of amino acid solution, amino acid solution, amino acid water-soluble fertilizer and application of amino acid water-soluble fertilizer

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preparation example Construction

[0020] The invention provides a preparation method of amino acid liquid, comprising the following steps:

[0021] (1) Extract the defatted soybean meal with water to obtain a soybean protein extract; the mass ratio of the defatted soybean meal to water is 1:(25~35);

[0022] (2) The soybean protein extract obtained in the step (1) is mixed with the Bacillus subtilis fermentation broth to ferment to obtain an amino acid solution; the amount of the Bacillus subtilis fermentation fluid accounts for 5% to 5% of the total volume of the soybean protein extract 10%; the number of effective viable bacteria in the Bacillus subtilis fermentation broth is more than or equal to 200 million / mL.

[0023] The present invention extracts the defatted soybean meal with water to obtain a soybean protein extract; the mass ratio of the defatted soybean meal to water is 1:(25-35), preferably 1:30. The present invention has no special limitation to the source of the defatted soybean meal, and conve...

Embodiment 1

[0036] Dry and pulverize 1000g defatted soybean meal, add distilled water according to the ratio of material to liquid 1:30, and add lye to adjust the pH to 9. Under the condition of 60°C and 60min, microwave 750W to extract soybean protein, centrifuge to remove impurities, collect supernatant 25L, and put Add 5% Bacillus subtilis fermentation broth at 30°C for 36 hours of fermentation treatment to obtain 450mL amino acid solution;

[0037] Add 1L of amino acid solution to 30g of ferrous sulfate heptahydrate, 80g of zinc sulfate heptahydrate, and 12g of boric acid, and stir for 1 hour at 25°C at a stirring speed of 500r / min to obtain an amino acid-containing water-soluble fertilizer.

Embodiment 2

[0039] Dry and pulverize 1000g defatted soybean meal, add distilled water according to the ratio of material to liquid 1:30, and add lye to adjust the pH to 9. Under the condition of 60℃ and 60min, microwave 750W to extract soybean protein, centrifuge to remove impurities, and collect 25L of supernatant , adding 5% Bacillus subtilis fermentation broth at 30°C for 48 hours to obtain 580mL amino acid solution;

[0040] Add 45g of EDTA-Fe, 65g of zinc sulfate heptahydrate, and 30g of boric acid to 1L of amino acid solution, and stir for 1 hour at 25°C at a stirring speed of 500r / min to obtain an amino acid-containing water-soluble fertilizer.

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Abstract

The invention provides a preparation method of an amino acid solution, the amino acid liquid, an amino acid water-soluble fertilizer and application of the amino acid water-soluble fertilizer, and relates to the technical field of agricultural fertilizer manufacturing. The preparation method comprises the following steps: (1) extracting defatted soybean meal with water to obtain a soybean proteinextract solution; a mass ratio of the defatted soybean meal to water is 1: (25 to 35); (2) mixing the soybean protein extract obtained in the step (1) with bacillus subtilis fermentation liquor for fermentation to obtain the amino acid solution; wherein the bacillus subtilis fermentation liquor has an amount accounting for 5% to 10% of a total volume of the soybean protein extract; and the bacillus subtilis fermentation liquor has an effective viable count greater than or equal to 200 million cells / mL. According to the preparation method, conversion rate of amino acid reaches more than 45%, and free amino acid content reaches more than 100 g / L.

Description

technical field [0001] The invention relates to the technical field of agricultural fertilizer production, in particular to a preparation method of amino acid liquid, amino acid liquid, amino acid water-soluble fertilizer and applications thereof. Background technique [0002] Due to people's continuous planting, long-term unreasonable use of chemical fertilizers, and repeated application of large amounts of water-soluble fertilizers, the soil is severely compacted, soil acidified, and soil salinized, resulting in an increasingly serious decline in soil fertility. The occurrence of soil-borne diseases eventually leads to the decline of crop quality. [0003] Resources such as soybean meal are rich in amino acids, and have a wide range of sources and low cost, but to a certain extent, they have not yet reached high-value utilization. Contents of the invention [0004] In view of this, the object of the present invention is to provide a preparation method of amino acid liqu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P13/04C07K1/14C05G1/00C12R1/125
CPCC05C11/00C07K1/145C12P13/04
Inventor 钱潘攀翟修彩王国玲杨风顺李永泉李肖宇林克明陈晓燕骆毛喜
Owner FUJIAN SANJU BIOLOGICAL SCI & TECH
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