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Haploidization in sorghum

A haploid, sorghum technology, applied in the field of breeding programs, can solve the problems of time-consuming and expensive

Pending Publication Date: 2019-12-06
KWS SAAT SE & CO KGAA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the process of cross-breeding relies on the continuous generation of homozygous sire and dam lines over several generations, which also makes the process of cross-breeding time-consuming and expensive, despite good results

Method used

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  • Haploidization in sorghum
  • Haploidization in sorghum

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Experimental program
Comparison scheme
Effect test

Embodiment

[0165] 1. Identification of patatin phospholipases as targets that confer haploid-inducible properties in sorghum

[0166] In the TILLING population, a sorghum plant capable of inducing haploidy with about 1% efficiency was found. Focused on various genes as potential targets for finding the genetic basis that confers this property in identified sorghum plants. The selection of genes was made on the basis of the fact that they are preferentially expressed in the reproductive organs of the plant and to some extent play a role in fertilization, since chromosome elimination can occur through defective or incomplete fertilization, e.g., deletion A haploid chromosome set is produced either by improperly or incorrectly transporting germ cells to the female ovule or by acting on energy metabolism in pollen.

[0167] According to preliminary studies in the context of the present invention, a mutated gene was then found which could be identified as Sorghum by means of the bioinformati...

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Abstract

The invention relates to Sorghum bicolor plants which, due to modifications to the genome relating to a pollen-specific expression of patatin phospholipase, are capable of inducing haploidy, thereby producing haploid progeny, and within short time inbred lines, i.e. homozygous paternal parent and maternal parent lines can be produced by way of chromosome duplication for hybrid breeding. The invention also relates to methods for producing transgenic and non-transgenic plant haploid inducers and for improving the induction power of plants.

Description

[0001] field of invention [0002] The present invention relates to the field of simplifying tedious breeding procedures by means of molecular biology methods, marker technology and genetic engineering. In particular, sorghum plants are provided which are capable of inducing haploidy by a modification in the genome involving preferably pollen-specific expression of patatin phospholipase, thereby producing haploid offspring and capable of doubling chromosomes in a short time Inbred lines for cross-breeding are produced in-house. In particular, provided are sorghum plants having mutations in patatin phospholipases, methods of producing and identifying these mutations or mutant plants and corresponding nucleic acid molecules encoding mutated patatin phospholipases as well as vectors and host cells, in particular containing said nucleic acid Molecular plant cells and haploid-producing plants derived from such plant cells, their progeny, cross products, inbred lines and their respec...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/82A01H1/08C12N9/18C07K14/415
CPCA01H1/08C07K14/415C12N9/18C12N15/8231C12N15/8287C12Y301/01002Y02A40/146C12N9/16C12Y301/04004
Inventor M·克洛伊贝尔-迈茨S·维克霍斯特C·博尔杜安M·奥祖诺娃
Owner KWS SAAT SE & CO KGAA
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