Method for making ascending aortic aneurysm animal model

An ascending aortic aneurysm, animal model technology, applied in medical science, veterinary instruments, veterinary surgery, etc., can solve the problems of affecting reliability, low tumor formation rate of animal models, long tumor formation time, etc., and achieve widespread promotion. Application, preparation method is simple and easy, and the effect of high tumor formation rate

Inactive Publication Date: 2019-12-10
SUZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In summary, the current methods used to make ATAA animal models have a low tumor formation rate and a long time for tumor formation, which affects the smooth progress of the experiment and the reliability of the final results.

Method used

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  • Method for making ascending aortic aneurysm animal model
  • Method for making ascending aortic aneurysm animal model
  • Method for making ascending aortic aneurysm animal model

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] The ascending aorta was narrowed surgically, and the ascending aortic aneurysm model was constructed by increasing the wall pressure and blood flow shear force. In the experiment, the ascending aorta of rats was constricted by using pad needles of different diameters (1.0 mm, 1.2 mm, 1.4 mm and 1.6 mm) to increase the wall pressure and shear force. The experimental steps are as follows:

[0035] (1) Purchase 8-week-old, 230-250g female Sprague Dawley rats from the Experimental Animal Center of Soochow University, fast for 12 hours before the operation, and drink normally. And weigh it, mark it.

[0036] (2) Rats were anesthetized by intraperitoneal injection of 10% chloral hydrate (3 mL / kg), and the hair from the suprasternal fossa to the chest of the rats was removed with depilatory cream.

[0037] (3) Disinfect the operating table with 75% alcohol, fix the rat on the operating table, select a 16-gauge arterial puncture trocar for oral and tracheal intubation, and co...

Embodiment 2

[0049] The operation was performed with a pad needle with a diameter of 1.4 mm. The operation steps were the same as in Example 1, except that the mice in the administration group were fed oral rehydration saline solution added with tramadol hydrochloride capsules (0.8 mg / mL) after the operation. (27.9 mg / mL), the rats in the non-administration group were fed with sterilized tap water, and the survival rate of the rats 5 days after the operation was counted. The results are as follows: Figure 4 , It can be seen that feeding mice with oral rehydration saline solution added with tramadol hydrochloride capsules can significantly improve the survival rate of mice.

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Abstract

The invention relates to a method for making an ascending aortic aneurysm animal model. The method comprises the following steps: (1) ligating the root of the ascending aorta of a mouse by using a cushion needle with the outer diameter of 1.0-1.6 mm; (2) feeding the mouse undergoing a ligation operation with an oral rehydration salt solution containing tramadol hydrochloride capsules. The making method is simple and feasible, can be widely applied and popularized, and is high in tumor forming rate and short in tumor forming time.

Description

technical field [0001] The invention specifically relates to a preparation method of an ascending aortic aneurysm animal model. Background technique [0002] Over the past 30 years, the prevalence of thoracic aortic aneurysm (TAA) has increased as the aging population has increased. The incidence of TAA in people over the age of 65 is approximately 3-4%, of which 60% are ascending aortic aneurysms (ATAA), predisposing patients to life-threatening complications such as aortic dissection or rupture. Current studies have shown that the formation mechanism of TAA is different from that of abdominal aortic aneurysm (AAA), and most thoracic aortic aneurysms are degenerative lesions. Pathologically, TAA exhibits matrix degeneration, loss of vascular smooth muscle, disruption of matrix elastic fibers, proteoglycan deposition, and infiltration of inflammatory cells (macrophages and T lymphocytes) in the aortic wall. During this process, accompanied by changes in the expression of m...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K67/02A61D1/00
CPCA01K67/02A61D1/00A01K2227/105A01K2267/03
Inventor 吴永冯建国王晓宇张燕霞余云生沈振亚
Owner SUZHOU UNIV
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