Application of polymorphism of retinoid X receptor alpha (RXRA) genes to adverse reactions of antituberculous drugs

A technology for adverse reactions and tuberculosis, applied in the direction of biochemical equipment and methods, microbial determination/testing, etc., can solve the problem of lack of susceptibility gene loci

Pending Publication Date: 2019-12-17
WEST CHINA HOSPITAL SICHUAN UNIV
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  • Abstract
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  • Application Information

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Problems solved by technology

In recent years, a large number of studies have shown that SNPs are the best candidate molecular markers for predicting the occurrence of ADR. Therefore, research on molecular markers of anti-ATDH and other types...

Method used

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  • Application of polymorphism of retinoid X receptor alpha (RXRA) genes to adverse reactions of antituberculous drugs
  • Application of polymorphism of retinoid X receptor alpha (RXRA) genes to adverse reactions of antituberculous drugs
  • Application of polymorphism of retinoid X receptor alpha (RXRA) genes to adverse reactions of antituberculous drugs

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Experimental program
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Embodiment 1

[0052] Mining of susceptibility SNP loci for liver injury induced by anti-tuberculosis drugs

[0053] 1 Inclusion of TB population in western China

[0054] A total of 1060 highly suspected tuberculosis patients who were treated at West China Hospital of Sichuan University from December 2016 to April 2018 were continuously included. According to the typical tuberculosis symptoms, microbiology and imaging results to confirm the diagnosis of tuberculosis patients, and excluding patients with liver disease and poor compliance during treatment or follow-up lost contact, this embodiment finally included 746 tuberculosis patients (see figure 1 ).

[0055] The inclusion and exclusion criteria are as follows:

[0056] Inclusion criteria: 1) have typical symptoms and signs of tuberculosis; 2) positive acid-fast stained smears or positive Mycobacterium tuberculosis culture or positive nucleic acid test; 3) imaging data showing typical evidence of active tuberculosis lesions; 4) diseas...

Embodiment 2

[0097] This embodiment provides a method for detecting the genotypes of rs55735339 and rs59211076, which is a small sequencing method, that is, a single base extension termination method. see the principle figure 2 Roughly as follows: in a reaction system containing sequencing enzymes, four kinds of fluorescently labeled ddNTPs, different length extension primers and PCR product templates close to the 5' or 3' end of the polymorphic site, the primer is extended by one base and then terminated, and finally The fluorescent signal is read by ABI3730 capillary electrophoresis, and the type of base incorporated can be known according to the color of the peak, so as to determine the genotype of the site. At the same time, multiplex PCR technology is used to detect multiple known sites.

[0098] The partial nucleotide sequences of the two SNP sites rs55735339 and rs59211076 on the RXRA gene are shown in Table 10 below.

[0099] The nucleotide sequences of the two SNP sites of rs557...

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Abstract

The invention discloses application of polymorphism of retinoid X receptor alpha (RXRA) genes to adverse reactions of antituberculous drugs, and relates to the field of screening of adverse reactionsof tuberculous drugs. The invention discloses application of the polymorphism of the RXRA genes to tuberculosis treatment, it is found that through detection of the polymorphism of the RXRA genes, therisk of generating the adverse reactions after tuberculous patients are treated by the antituberculous drugs can be predicted, certain support is provided for avoiding the adverse reactions caused bythe related antituberculous drugs, and certain guidance is also provided for drug use of the tuberculous patients.

Description

technical field [0001] The invention relates to the field of tuberculosis drug adverse reaction screening, in particular to the application of RXRA gene polymorphisms in anti-tuberculosis drug adverse reactions. Background technique [0002] Tuberculosis (TB) is a major infectious disease that seriously endangers human health. According to the World Health Organization (WHO) Global Tuberculosis Annual Report, in 2017, about 10 million people in the world were newly infected with tuberculosis, and about 889,000 people were newly infected with tuberculosis in China, making my country the second-highest burden of tuberculosis in the world. Tuberculosis ranked second in the number of legal infectious diseases in my country in 2017, and the second in mortality. The situation of anti-tuberculosis treatment is still very serious. The cornerstone of tuberculosis treatment is the WHO-recommended standardized anti-tuberculosis regimen: rifampicin (R), isoniazid (H), ethambutol (E) an...

Claims

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Application Information

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IPC IPC(8): C12Q1/6883
CPCC12Q1/6883C12Q2600/156C12Q2600/172C12Q2600/106
Inventor 焦琳
Owner WEST CHINA HOSPITAL SICHUAN UNIV
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