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Trace Pb Based on Wheatstone Bridge Smart Microfluidic Chip 2+ Detection method

A technology of microfluidic chips and detection methods, applied in chemical instruments and methods, measuring devices, flow characteristics, etc.

Active Publication Date: 2021-02-19
四川大川精密科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, how to further effectively convert and amplify the small changes in smart hydrogels caused by trace amounts of analytes into easy-to-read and easy-to-detect output signals to achieve ultra-sensitive quantitative detection of even more trace analytes is still a challenge. big challenge

Method used

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  • Trace Pb Based on Wheatstone Bridge Smart Microfluidic Chip <sup>2+</sup> Detection method
  • Trace Pb Based on Wheatstone Bridge Smart Microfluidic Chip <sup>2+</sup> Detection method
  • Trace Pb Based on Wheatstone Bridge Smart Microfluidic Chip <sup>2+</sup> Detection method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0054] In this embodiment, a structure based on a Wheatstone bridge-type intelligent microfluidic chip is provided. The structural schematic diagram of the intelligent microfluidic chip is as follows Figure 1~3As shown, the smart microfluidic chip includes a substrate 1, a gel sheet 1-11 and a transparent cover 2. The substrate 1 is provided with a first bridge arm microchannel 1-1, a second bridge arm microchannel 1-2, a second bridge arm microchannel 1-2, and a second bridge arm microchannel 1-2. Three bridge arm microchannels 1-3, fourth bridge arm microchannels 1-4, middle bridge microchannels 1-5, sample injection microchannels 1-6, indicator solution microchannels 1-7 and sample outlet microchannels 1-8 .

[0055] The microchannel 1-1 of the first bridge arm and the microchannel 1-4 of the fourth bridge arm, the microchannel 1-2 of the second bridge arm and the microchannel 1-3 of the third bridge arm are symmetrically arranged on the left and right axis, and the microc...

Embodiment 2

[0064] In this example, based on the structure of the Wheatstone bridge-type intelligent microfluidic chip, the preparation method of each microchannel on the substrate and the fixed column, and the method of setting the gel sheet in the fourth bridge arm microchannel and Example 1 Basically the same, the only differences are:

[0065] First bridge arm microchannel 1-1, second bridge arm microchannel 1-2, third bridge arm microchannel 1-3, fourth bridge arm microchannel 1-4, middle bridge microchannel 1-5, sample injection The depths of microchannels 1-6, indicator liquid microchannels 1-7 and sample outlet microchannels 1-8 are all 30 μm; the first bridge arm microchannel 1-1, the second bridge arm microchannel 1-2, the third bridge arm The width of the arm microchannel 1-3 and the fourth bridge arm microchannel 1-4 is 600 μm; the width of the middle bridge microchannel 1-5 is 1300 μm, and the length is 4000 μm; the width of the indicator liquid microchannel 1-7 is 100 μm; t...

Embodiment 3

[0068] In this example, based on the structure of the Wheatstone bridge-type intelligent microfluidic chip, the preparation method of each microchannel on the substrate and the fixed column, and the method of setting the gel sheet in the fourth bridge arm microchannel and Example 1 Basically the same, the only differences are:

[0069] First bridge arm microchannel 1-1, second bridge arm microchannel 1-2, third bridge arm microchannel 1-3, fourth bridge arm microchannel 1-4, middle bridge microchannel 1-5, sample injection The depths of microchannels 1-6, indicator liquid microchannels 1-7 and sample outlet microchannels 1-8 are all 80 μm; the first bridge arm microchannel 1-1, the second bridge arm microchannel 1-2, the third bridge arm The width of the arm microchannel 1-3 and the fourth bridge arm microchannel 1-4 is 800 μm; the width of the middle bridge microchannel 1-5 is 2000 μm, and the length is 5000 μm; the width of the indicator liquid microchannel 1-7 is 200 μm; t...

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Abstract

The invention provides a trace Pb based on Wheatstone bridge type intelligent microfluidic chip 2+ The detection method is to place the intelligent microfluidic chip on the hot stage, connect the sample inlet and the indicator liquid inlet of the intelligent microfluidic chip to the constant current delivery device through pipe fittings, and the detection steps are as follows: ①Detect the blank sample , after the interface of the indicator liquid in the smart microfluidic chip is stable, measure the width of the indicator liquid at the inlet of the first bridge arm microchannel; ② detect the standard sample and determine the Pb 2+ The conversion relationship between the concentration and the width change rate of the indicator solution; ③ detect the sample to be tested and calculate the Pb in the sample to be tested 2+ concentration. This method will Pb 2+ The concentration signal has undergone multiple conversion and double amplification, realizing the detection of trace Pb 2+ Ultra-sensitive quantitative detection, effectively reducing Pb 2+ detection limit.

Description

technical field [0001] The invention belongs to the field of analysis and detection of trace lead ions, and relates to a trace Pb based on Wheatstone bridge type intelligent microfluidic chip 2+ Detection method. Background technique [0002] Timely detection of trace chemical and biological substances harmful to the environment and human health plays a vital role in disease diagnosis and environmental protection. Pb 2+ It is one of the most abundant toxic heavy metal pollutants, because it is easy to accumulate in the human body, even trace amounts of Pb 2+ It can also cause kidney and nervous system damage and brain disorders, especially in developing children. According to the Chinese national standard (GB-25466-2010) and the requirements of the World Health Organization (WHO), Pb in drinking water 2+ The concentration cannot exceed 4.83×10 -8 mol / L. Therefore, timely and sensitive detection or monitoring of trace Pb in drinking water 2+ is of great significance. ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N11/00B01L3/00
CPCB01L3/5027B01L3/502707G01N11/00
Inventor 彭减汪伟褚良银林硕赵宁苏瑶瑶谢锐巨晓洁刘壮
Owner 四川大川精密科技有限公司
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