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A probiotic composition for intervening inflammatory bowel disease and its screening method and application

A technology of inflammatory bowel disease and screening method, applied in the field of biomedicine, can solve problems such as difficult host adaptation, limited scope of application, poor colonization ability, etc., achieve excellent universality and comprehensive information, avoid blindness, purpose strong effect

Active Publication Date: 2022-03-25
君维安(武汉)生命科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, currently targeted therapeutic approaches with probiotics have not been intensively studied in IBD
[0005] Chinese Patent No. CN201910495866.7 discloses the use of a compound probiotic agent for the preparation of products for the treatment of ulcerative colitis. The selected probiotics of Mycobacterium V9, Lactobacillus plantarum P-8 and Lactobacillus plantarum C2 are not all derived from the human body. After entering the host body, it is difficult to adapt to the host, the colonization ability is poor, and the efficacy is limited; The scope is limited and cannot be aimed at the physique of different Chinese people

Method used

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  • A probiotic composition for intervening inflammatory bowel disease and its screening method and application
  • A probiotic composition for intervening inflammatory bowel disease and its screening method and application
  • A probiotic composition for intervening inflammatory bowel disease and its screening method and application

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Experimental program
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Effect test

Embodiment 1

[0043] A method for screening a probiotic composition for intervening inflammatory bowel disease, comprising the following steps:

[0044] S1. Construct a human intestinal microbiome database, which integrates intestinal flora metagenomic sequences and species annotation information from a wide range of hosts; specific operations are as follows:

[0045] S11. Search and download the metagenomic raw data of normal people and patients with inflammatory bowel disease in the biological information database; in this embodiment, the biological information database is the online warehouse GMrepo of the human intestinal microbiome. You can choose NCBI, etc.;

[0046] S12, use Trimmomatic software to filter and quality control the raw data, trim and remove adapters and low-quality sequences in the raw data, require 10% quantiles of all positions to be greater than 30, and use Fastqc software to evaluate the quality control. data to obtain high-quality sequences;

[0047] S13, using M...

Embodiment 2

[0060] Embodiment 2 Animal in vivo verification experiment

[0061]Preparation of probiotic suspension: purchase probiotics, inoculate them in liquid medium before use, and incubate at 37°C for 16 hours. After separation (3000rpm, 8min, 4°C), discard the supernatant, and adjust the bacteria sludge with sterile normal saline. The suspension concentration is 10 12 CFU / mL for use.

[0062] Experimental grouping: blank control group, DSS model group, probiotics group A, probiotics group B, and probiotics group C and comparative examples, the ratio of probiotics is shown in Table 1 below.

[0063] Table 1 The proportion table of different groups of probiotics

[0064]

[0065] Animal experiments: Experimental mice (4 weeks old, male, 18-22g) were adaptively fed for one week in a constant temperature (22°C), constant humidity, and 12h day-night cycle environment before the formal experiment. During the experiment, they were free to drink and eat. Dextran sodium sulfate (DSS) e...

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Abstract

The invention provides a probiotic composition for intervening inflammatory bowel disease, its screening method and application, and relates to the technical field of biomedicine. The probiotic composition includes: Bifidobacterium animalis, Bifidobacterium bifidum, Bifidobacterium longum, Bifidobacterium scardovii, Clostridium butyricum , Lactobacillus brevis, Lactobacillus delbrueckii, Oxalobacter formigenes, Lactobacillus acidophilus, Enterococcus faecalis; the present invention comprehensively considers a number of influencing factors, in addition to supplementing the specific disease-treating bacteria that antagonize inflammatory enteritis, it also adds probiotics that protect the intestinal mucosa, Probiotics that improve immunity and probiotics that can reduce inflammatory responses can improve or treat inflammatory bowel disease by enhancing intestinal mucosal barrier function, regulating intestinal mucosal immunity, and restoring the composition of intestinal microbial flora. Better curative effect.

Description

technical field [0001] The invention relates to the technical field of biomedicine, in particular to a probiotic composition for intervening inflammatory bowel disease, a screening method and application thereof. Background technique [0002] Inflammatory bowel disease (Inflammatory Bowel Disease, referred to as IBD) is a group of unexplained chronic intestinal inflammatory diseases, the main clinical manifestations are abdominal distension, abdominal pain, diarrhea, mucus bloody stool, mucous membrane pustule; including Crohn's disease (CD) and Ulcerative colitis (UC), characterized by persistent non-specific intestinal inflammation, usually recurs and persists, and there is still no specific clinical treatment. In recent years, more and more studies have reported that intestinal flora and its metabolites, host gene susceptibility, and intestinal mucosal immunity are jointly involved in the pathogenesis of IBD, and it has been confirmed that the composition of intestinal fl...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20G16B40/20G16B35/20G16B50/30G16B20/00A61K35/742A61K35/745A61K35/747A61K35/741A61P29/00A61P1/00C12R1/01C12R1/145C12R1/24C12R1/225C12R1/23
CPCC12N1/20G16B40/20G16B35/20G16B50/30G16B20/00A61K35/742A61K35/745A61K35/747A61K35/741A61P29/00A61P1/00A61K2300/00
Inventor 陈卫华江浦滋吴思成聂庆庆刘智
Owner 君维安(武汉)生命科技有限公司
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