Molecular marker for identifying homozygous or heterozygous types of eriobotrya japonic lindl yellow-flesh character genes and application of molecular marker

Abstract

The invention discloses a molecular marker for identifying homozygous or heterozygous types of eriobotrya japonic lindl yellow-flesh character genes and application of the molecular marker. When a variety of an eriobotrya japonic lindl yellow flesh character gene type is homozygous, a nucleotide sequence of the molecular marker is as shown in SEQ ID NO. 1; when the variety of the eriobotrya japonic lindl yellow flesh character gene type is heterozygous, a nucleotide sequence of the molecular marker is as shown in SEQ ID NO. 1-2. Through the molecular marker, the variety of the eriobotrya japonic lindl yellow-flesh character gene types can be accurately identified in advance. For the breeding of a new variety of white-flesh eriobotrya japonic lindl, a heterozygous type and a heterozygous type can be selected or the heterozygous type is hybridized with the white-flesh type during the selection and mating of cross parents, and the white-flesh character must appear in filial generation groups, and the new variety of white-flesh eriobotrya japonic lindl may be obtained, the breeding efficiency is improved, and the breeding cost is reduced.

Description

Technical field [0001] The present invention relates to the technical field of fruit tree genetic breeding, in particular to a molecular marker for identifying homozygous or heterozygous types of loquat yellow flesh trait genes and its application. Background technique [0002] Loquat (Erobotrya Japonic Lindl.) is native to China and has a cultivation history of more than 2000 years. It is one of the important early spring fruits in my country. The cultivation area and total output of loquat in my country rank first in the world. The main cultivation areas are concentrated in the Yangtze River basin and the provinces south of the Yangtze River. Among them, Zhejiang, Fujian and Sichuan have larger cultivation areas and have successively become the leader of my country's loquat industry. Since the 1990s, Golden Pine and others first used hybrid breeding technology to bred China's first hybrid loquat variety'Zaozhong 6', and thus established a loquat hybrid breeding technology syste...

AI Technical Summary

Problems solved by technology

At present, DNA markers are widely used in loquat research, such as evaluation of genetic diversity, genetic linkage map and germplasm identification, but there are few studies on molecular markers of loquat fruit color. Some specific markers can distinguish 'Guifei', 'Xinbai 1' and 'Xinbai 8'

However, for loquat varieties with yellow flesh, there are no reports of molecular markers that can distinguish the genetic tendency of fruit color traits in offspring of yellow flesh loquats.

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