A method and cultivation method for directly obtaining distant hybrids of Congo wild sesame and cultivated species

A technology of remote hybrids and wild sesame, which is applied in cultivation, plant cultivation, botanical equipment and methods, etc., can solve the problems of inability to obtain sesame distant hybrid plants, promotion and utilization of sesame hybrid plants, and improve hybrid compatibility Effect

Active Publication Date: 2021-05-25
江西省农业科学院作物研究所
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  • Abstract
  • Description
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  • Application Information

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Problems solved by technology

[0005] Therefore, the technical problem to be solved in the present invention is to overcome the defects that the conventional hybridization method cannot obtain the sesame distant hybrid plant, and the immature embryo rescue technique is difficult to widely popularize and utilize the method for obtaining the sesame hybrid plant in the prior art, thereby A method and cultivation method for directly obtaining distant hybrids of wild sesame and cultivated species are provided

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  • A method and cultivation method for directly obtaining distant hybrids of Congo wild sesame and cultivated species
  • A method and cultivation method for directly obtaining distant hybrids of Congo wild sesame and cultivated species
  • A method and cultivation method for directly obtaining distant hybrids of Congo wild sesame and cultivated species

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Embodiment

[0043] The present embodiment provides a method for obtaining a distant hybrid of Congo wild sesame and a cultivar, and a cultivation method of the hybrid, which specifically includes the following steps:

[0044] Congo wild sesame was used as the female parent, and the cultivar Zhongzhi 11 was used as the male parent.

[0045] Planting of both parents: the female parent was sown on May 25, 2017, and the male parent 11 was sown on June 6, 2017. Wherein, the female parent's row spacing is 60cm, and the plant spacing is 40cm; the male parent's row spacing is 40cm, and the plant spacing is 20cm.

[0046] Collection and short-term storage of pollen from the male parent: After the parents entered the full flowering period, at 7:00 am on July 25, 2017, the corolla (including stamens) that opened and pollen began to be released from the male parent was collected and placed on a damp mat. In the sealed box of the filter paper, the humidity is 80%, and the sealed box is placed in a lo...

experiment example

[0063] Morphological and molecular marker identifications were carried out on the progeny plants of the above experimental examples.

[0064] Morphological identification of progeny plants:

[0065] 25 days after transplanting the seedlings, the progeny plants entered the bud flowering stage, and compared and observed the traits such as leaves, stems, corolla color, nectary size, and pollen fertility of the parents and the hybrid progeny plants in the examples and comparative examples, as shown in Table 1. , the plant growth of Experimental Example 1 is shown in the attached figure 1 .

[0066] The configuration of 1% acetic acid magenta staining solution: Take 45ml of glacial acetic acid, add 55ml of distilled water, slowly add 1g of magenta after boiling, stir well, add 1 rust nail, boil for 10 minutes, filter after cooling, and store in a brown bottle.

[0067] The specific steps of pollen fertility observation are as follows:

[0068] Take the pollen and place it on a g...

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Abstract

The method and cultivation method for directly obtaining distant hybrids of Congo wild sesame and cultivars provided by the present invention, the method for obtaining the distant hybrids comprises the following steps: using Congo wild sesame as the female parent and sesame cultivars as the male parent for artificial hybridization, Conventional field management, the harvested seeds are the distant hybrids; wherein, the artificial hybridization includes artificial pollination for more than two consecutive days before and after the flowering of the female parent. Real distant hybrids can be obtained by limiting the time of repeated pollination. It overcomes the shortcomings that conventional hybridization methods cannot directly obtain sesame distant hybrid plants, and the method of using immature embryo rescue technology to obtain sesame hybrid plants is difficult to be widely promoted and utilized; it lays the foundation for the transfer and utilization of Congo wild sesame excellent genes , providing technical support for perfecting sesame distant hybrid breeding technology.

Description

technical field [0001] The invention relates to the technical field of biological breeding, in particular to a method and a cultivation method for obtaining distant hybrids of wild sesame and cultivated species. Background technique [0002] Sesame (S.indicum L., 2n=26) belongs to the genus Linaceae, is one of the ancient oil crops, and is also a traditional high-quality oil crop in my country; and sesame seeds are rich in unsaturated fatty acids and proteins, as well as sesame Sesamin, sesamol, vitamin E and other natural antioxidant substances, therefore, sesame is also a health food, with high nutritional value and broad consumer groups, it is an indispensable part of many traditional foods in my country raw materials. [0003] However, due to the long-term evolution and artificial selection of sesame cultivars, many good genes have been eliminated, resulting in narrower and narrower genetic basis among breeding parents, and poorer genetic diversity; Susceptible to enviro...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H1/02A01G22/00
CPCA01H1/02A01G22/00
Inventor 孙建乐美旺颜廷献叶艳英饶月亮颜小文周红英梁俊超
Owner 江西省农业科学院作物研究所
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