Takifugu obscurus SNP molecular markers and application thereof in genetic breeding

Abstract

The invention discloses takifugu obscurus SNP molecular markers and application thereof in genetic breeding. The takifugu obscurus SNP molecular markers includes the SNP molecular marker TGF120 and the SNP molecular marker CIRP41, wherein the SNP molecular marker TGF120 is related to weight and standard head length, and the SNP molecular marker CIRP41 is related to cold resistance; amino acid sequences where the SNP molecular markers are located are shown in SEQ ID NO:1 and SEQ ID NO:2 respectively; the TGF120 is located at a 120th site, namely an m with a corresponding base of C or A, of thesequence shown in SEQ ID NO:1; and the CIRP41 is located at a 41th site, namely a y with a corresponding base of A or T, of the sequence shown in SEQ ID NO:2. By adopting the takifugu obscurus SNP molecular markers, takifugu obscurus breeding parents which grow well and resist low temperature can be effectively selected, and thus tetraodontidae variety improving and industry developing are promoted. The invention further provides a takifugu obscurus genetic quality judgement system. Therefore, workloads of breeding selection can be reduced, and the work efficiency is improved.

Description

Technical field [0001] The invention belongs to aquatic breeding technology, and particularly relates to the SNP molecular marker of obscured puffer and its application in genetic breeding. Background technique [0002] Takifugu fasciatus (Takifugu fasciatus), commonly known as pufferfish, is a valuable economic fish of the order Bonefish, Radial fish, and Takifugu. It is widely distributed in coastal areas and inland rivers of China, Japan and the Korean Peninsula. With the development of industrial technology, fishing technology and efficiency have been rapidly improved. Uncontrolled fishing and emission pollution have caused the population of obscured puffer fish to decline rapidly. The fishing flood of wild obscured puffer has basically disappeared, seriously threatening ecological diversity and Stability has also caused a blow to the trade economy of the obscured puffer. Therefore, artificial selection of rare fish species such as obscured puffer fish to alleviate the contr...

AI Technical Summary

Problems solved by technology

With the development of the artificial breeding process of puffer obscurus, the breeding of its fine species has gradually entered the right track, but there are still many problems: although the appropriate breeding density and sufficient bait have been ensured, there are still many individuals that appear similar to Other individuals in the same period have a large difference in growth, and this is mostly reflected in body weight; although some individuals have little difference in weight from other individuals, their trunks are short, which means that there are not many edible parts; under natural conditions, puffer obscurus The natural water bodies in which they live are mostly seawater. After entering rivers and artificial breeding environments, they may be stimulated by the low temperature environment and cause mass death. temperature will cause a huge loss

For puffer obscurus, no related studies and SNP reports of these two genes have been published

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