SNP Molecular Markers in Puffer Puffer Obscura and Its Application in Genetic Breeding

Abstract

The invention discloses a fugu obscurus SNP molecular marker and its application in genetic breeding. The published SNP molecular markers of Pufferfish obscura include body weight, standard head length-related SNP molecular marker TGF120 and cold resistance-related SNP molecular marker CIRP41, and their base sequences are shown as SEQ ID NO: 1 and SEQ ID NO: 2, the TGF120 is located at the 120th m of the sequence shown in SEQ ID NO:1, and the base is C or A, and the CIRP41 is located at the 41st y of the sequence shown in SEQ ID NO:2, and the base is A or T. The two molecular markers of the invention can effectively realize the selection of breeding parents of puffer puffers with good growth and low temperature resistance, and promote puffer puffer breeding and industrial development. The invention also provides a system for judging the genetic quality of puffer puffer, which can reduce the workload on breeding selection and improve work efficiency.

Description

technical field [0001] The invention belongs to aquatic breeding technology, and in particular relates to the SNP molecular marker of puffer obscurus and its application in genetic breeding. Background technique [0002] Fugu (Takifugu fasciatus), commonly known as puffer fish, belongs to the class of bony fishes, subclass of ray-finned fishes, and rare economic fish of the order of fuguformes. It is widely distributed in coastal areas and inland rivers of China, Japan and the countries of the Korean Peninsula. With the development of industrial technology, fishing technology and efficiency have been rapidly improved. Uncontrolled fishing and pollution discharge have caused the population of puffer puffer to decline rapidly. The fishing season of puffer puffer obscurus has basically disappeared, seriously threatening ecological diversity and Stability has also caused a blow to the pufferfish trade economy. Therefore, artificial breeding of rare fish species such as Puffer o...

AI Technical Summary

Problems solved by technology

With the development of the artificial breeding process of puffer obscurus, the breeding of its fine species has gradually entered the right track, but there are still many problems: although the appropriate breeding density and sufficient bait have been ensured, there are still many individuals that appear similar to Other individuals in the same period have a large difference in growth, and this is mostly reflected in body weight; although some individuals have little difference in weight from other individuals, their trunks are short, which means that there are not many edible parts; under natural conditions, puffer obscurus The natural water bodies in which they live are mostly seawater. After entering rivers and artificial breeding environments, they may be stimulated by the low temperature environment and cause mass death. temperature will cause a huge loss

For puffer obscurus, no related studies and SNP reports of these two genes have been published

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