Attractant formula for controlling Holotrichia oblita adults

A large black gill beetle, attractant technology, applied in the direction of attracting pests, chemicals for biological control, pest control, etc., can solve the problems of high residual characteristics, hidden damage methods, high toxicity, etc., to achieve prevention and control The effect is good, the production cost is low, and the preparation is simple

Inactive Publication Date: 2020-03-17
INST OF PLANT PROTECTION CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Because grubs are soil-dwelling and cause hidden damage, in the past they mainly relied on long-lasting chemical pesticides for control, such as 666, aldicarb, etc. These pesticides have high toxicity and high residue characteristics, and have long been banned in China. disabled
And with the abuse of chemical pesticides, these pesticides have caused a series of serious social problems

Method used

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  • Attractant formula for controlling Holotrichia oblita adults
  • Attractant formula for controlling Holotrichia oblita adults
  • Attractant formula for controlling Holotrichia oblita adults

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Example 1 Screening of Pheromone Monomers to the Optimum Attractive Concentration of North China Black Beetle

[0019] The optimal concentration of 5 kinds of plant volatile monomers to attract adults of the North China black gill beetle is known, including dibutyl phthalate, cinnamaldehyde, cis-3-hexenyl acetate, 1-hexanol, The concentrations of cis-3-hexenyl isobutyrate were 0.11 μg / μL, 0.33 μg / μL, 1.00 μg / μL, 0.33 μg / μL, 3.00 μg / μL, respectively.

[0020] The concentration of attractant pheromone monomers in the North China giant beetle was determined respectively. Aggregation pheromone cis-9-octadecenal and cis-9-octadecenyl acetate, sex pheromone glycine methyl ester 3 compounds were diluted to 0.11μg / ml and 0.33μg / ml with paraffin oil as solvent , 1μg / ml, 3μg / ml, 9μg / ml and other 5 concentration gradients, and the best attractant concentration was found through the behavioral response test of tropism. According to the nocturnal habit of the test insects, the tes...

Embodiment 2

[0028] Embodiment 2 prepares attractant

[0029] A total of 17 groups of binary attractant formulas were obtained by mixing the selected plant volatiles, aggregation pheromones and sex pheromones of the North China giant black beetle. According to the results of the best attractant concentration of each compound monomer to the test insects, the content ratio of each component in the formula is determined, so that the concentration of the two compound monomers in the formula is the best attractant concentration. With paraffin oil as solvent, prepare 100mL of each formulation. The composition and content of each formula are shown in Table 3.

[0030] table 3

[0031]

[0032]

Embodiment 3

[0033] Embodiment 3 test insect is measured to the antenna potential of different formulas

[0034] Select an active test insect from the insect breeding box, gently press the base of its antennae with tweezers to open the three gills of the antennae, quickly cut off the antennae from the base, and fix it on both ends of the electrodes with conductive glue, between the two electrodes The tentacles should not be glued, otherwise it will affect the test results. Use a pipette gun to take 20 μL of the formula solution and apply it evenly on a 4cm×3mm filter paper strip, then fold the filter paper strip in half longitudinally, put it into a Pasteur tube, seal it, and take it for the test. During the test, it is necessary to ensure that the laboratory is clean and free of peculiar smell, and there are no factors that affect the test results such as personnel walking. After debugging the software, the antennae can be stimulated with odor after the baseline is stable. The flow rate...

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Abstract

The invention discloses an attractant formula for controlling Holotrichia oblita adults in the technical field of biological control of harmful organisms. The attractant formula is composed of cis-3-hexenyl acetate and cis-9-octadecenyl acetate in a ratio of 100:11.3. The attractant formula has good control effect on Holotrichia oblita, and will not cause resistance in Holotrichia oblita. The preparation process is simple, the manufacturing cost is low, and the attractant is harmless to humans and animals, is environmentally friendly, and is of great significance for the comprehensive controlof the Holotrichia oblita.

Description

technical field [0001] The invention belongs to the technical field of biological control of harmful organisms, and in particular relates to an attractant formula for preventing and controlling adults of the North China black gill beetle. Background technique [0002] The North China giant black beetle, Holotrichia oblita, belongs to the family Coleoptera and is an important underground pest in the arid region of North China. Its larvae (grubs) feed on the underground parts of plants, often causing severe yield losses in peanuts, soybeans, and potatoes. Because grubs are soil-dwelling and cause hidden damage, in the past they mainly relied on long-lasting chemical pesticides for control, such as 666, aldicarb, etc. These pesticides have high toxicity and high residue characteristics, and have long been banned in China. disabled. And with the abuse of chemical pesticides, these pesticides have caused a series of serious social problems. There is a new measure to reduce the...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N37/10A01N35/02A01N37/02A01N37/44A01N31/02A01P19/00
CPCA01N31/02A01N35/02A01N37/02A01N37/10A01N37/44
Inventor 尹姣李克斌李晓峰张帅梅向东伊洛森
Owner INST OF PLANT PROTECTION CHINESE ACAD OF AGRI SCI
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