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CircRNA markers for diagnosing poorly differentiated adenocarcinoma of stomach and application thereof

A technique for gastric poorly differentiated adenocarcinoma and markers, which is applied in the field of circRNA markers for the diagnosis of gastric poorly differentiated adenocarcinoma, and achieves the effects of broad clinical application prospects, stable results and high sensitivity.

Pending Publication Date: 2020-03-20
潘文胜
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

Studies have shown that circRNA can regulate gene expression at multiple levels such as transcription and post-transcription, and plays an important role in regulating various physiological and pathological processes of the body. Recent studies have also found that circRNA is involved in the occurrence and development of tumors. Some circRNA markers with diagnostic potential have been discovered in gastric cancer, but no circRNA markers with diagnostic potential in gastric poorly differentiated adenocarcinoma have been reported

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  • CircRNA markers for diagnosing poorly differentiated adenocarcinoma of stomach and application thereof
  • CircRNA markers for diagnosing poorly differentiated adenocarcinoma of stomach and application thereof
  • CircRNA markers for diagnosing poorly differentiated adenocarcinoma of stomach and application thereof

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Embodiment 1

[0023] 1. Clinical Sample Collection

[0024] Case population and clinical specimens: Clinical information of 40 patients who underwent radical gastrectomy in Zhejiang Provincial People's Hospital from October 2017 to December 2018, as well as corresponding gastric cancer surgical specimens and paired paracancerous surgical specimens, all Once the tissue samples were taken out of the body, the blood was washed with PBS and immediately stored in a liquid nitrogen tank, and then the samples were stored in a -80°C biospecimen bank until use. of tumor cells,

[0025] 2. Total RNA extraction and quantification, using TRIzol Reagent kit to extract tissue total RNA from gastric cancer and paracancerous tissues. The extracted total RNA was then quantified using an ultra-micro-volume ultraviolet-visible spectrophotometer (NanoDrop ND2000).

[0026] The five groups of hsa_circ_0102434, hsa_circ_0005505, hsa_circ_0077837, hsa_circ_0072309, and hsa_circ_0070033 provided by the present i...

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Abstract

The invention relates to circRNA markers for diagnosing poorly differentiated adenocarcinoma of a stomach. The markers comprise five circRNAs, i.e., hsa_circ_0102434 shown in SEQ ID NO: 1, hsa_circ_0005505 shown in SEQ ID NO: 2, hsa_circ_0077837 shown in SEQ ID NO: 3, hsa_circ_0072309 shown in SEQ ID NO: 4 and hsa_circ_0070033 shown in SEQ ID NO: 5. The circRNA markers all have a certain diagnosisvalue to diagnosis on the poorly differentiated adenocarcinoma of the stomach, AUC, sensitivity and specificity of combined diagnosis all are superior to those of single circRNA assaying, in an AUC curve, the area is the maximum, the sensitivity and specificity can reach 90% or more, and thus, the circRNA markers are relatively-reliable specific biomarkers for diagnosing the poorly differentiatedadenocarcinoma of the stomach.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a group of circRNA markers for the diagnosis of gastric poorly differentiated adenocarcinoma and applications thereof. Background technique [0002] In general, adenocarcinoma with few glandular structures in the histological extent is diagnosed as poorly differentiated adenocarcinoma (Poorly differentiated adenocarcinoma). In gastric cancer (Gastric cancer), poorly differentiated adenocarcinoma mainly occurs in female and young patients, including signet ring cell carcinoma and low adhesion adenocarcinoma. The current standard treatment for poorly differentiated gastric adenocarcinoma includes surgical resection, chemotherapy, and targeted therapy. Gastric poorly differentiated adenocarcinoma has a high degree of malignancy and a poor prognosis. Although the level of diagnosis and treatment has gradually improved, the 5-year survival rate of patients with poorly differ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6886C12N15/11
CPCC12Q1/6886C12Q2600/158C12Q2600/178
Inventor 潘文胜张晨静王婧雅耿晓歌
Owner 潘文胜
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