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Hierarchical pore carbon microsphere carrier for immobilized enzyme and its preparation method and application

A technology of hierarchical pore carbon and immobilized enzymes, applied in the direction of immobilization on or in inorganic carriers, biochemical equipment and methods, carbon preparation/purification, etc., can solve blocking, unfavorable macromolecular enzyme adsorption enzymes and substrates Contact and other problems, to achieve the effect of cheap and easy-to-obtain raw materials, excellent immobilization effect, stability and reusability

Active Publication Date: 2021-06-15
SHENYANG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] Mesoporous carbon materials have good biocompatibility, non-toxicity, stable physical and chemical properties and rich pore structure, which can provide multi-point adsorption and storage space for enzyme molecules, while a single mesoporous structure is not conducive to the adsorption and storage of macromolecular enzymes. Enzyme-substrate contact, possibly even blocking

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  • Hierarchical pore carbon microsphere carrier for immobilized enzyme and its preparation method and application
  • Hierarchical pore carbon microsphere carrier for immobilized enzyme and its preparation method and application
  • Hierarchical pore carbon microsphere carrier for immobilized enzyme and its preparation method and application

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Embodiment 1

[0056] A preparation method for a hierarchically porous carbon microsphere carrier for immobilized enzymes, the described a kind of hierarchically porous carbon microsphere carrier for immobilized enzymes, the average pore diameter of its mesopore structure is 15nm, and the macroporous structure is 100nm, specific surface area 630m 2 / g, the macroscopic diameter of the hierarchical porous carbon microsphere carrier for immobilized enzyme is 2mm, and the pore size distribution figure for the hierarchical porous carbon microsphere carrier of immobilized enzyme is as follows figure 1 As shown, the microscopic morphology is shown in figure 2 As shown, there are a large number of carboxyl groups on the surface and pore walls of the hierarchical porous carbon microsphere carrier used for immobilizing enzymes after activation, and the specific preparation method is as follows:

[0057] Step 1: Dissolve

[0058] Weigh each material in molar ratio; mix ethylene glycol and distilled ...

Embodiment 2

[0069] A preparation method for a hierarchically porous carbon microsphere carrier for immobilized enzymes, the described a kind of hierarchically porous carbon microsphere carrier for immobilized enzymes, the average pore diameter of its mesopore structure is 25nm, and the macroporous structure is 150nm, the specific surface area is 393m 2 / g, the macroscopic diameter of the hierarchical porous carbon microsphere carrier for immobilized enzyme is 2mm, and the pore size distribution figure for the hierarchical porous carbon microsphere carrier of immobilized enzyme is as follows image 3 As shown, there are a large number of carboxyl groups on the activated hierarchical porous carbon microsphere carrier for immobilized enzymes, and the specific preparation method is as follows:

[0070] Step 1: Dissolve

[0071] Weigh each material in molar ratio; mix ethylene glycol and distilled water to obtain a mixed system of ethylene glycol and distilled water; wherein, in molar ratio, ...

Embodiment 3

[0082] A preparation method for a hierarchically porous carbon microsphere carrier for immobilized enzymes, the described a kind of hierarchically porous carbon microsphere carrier for immobilized enzymes, the average pore diameter of its mesopore structure is 20nm, and the macroporous structure is 200nm, the specific surface area is 313m 2 / g, the macroscopic diameter of the hierarchical porous carbon microsphere carrier used for immobilized enzyme is 3mm, and the pore size distribution of the hierarchical porous carbon microsphere carrier used for immobilized enzyme is as follows Figure 4 As shown, the microscopic morphology is shown in Figure 5 As shown, there are a large number of carboxyl groups on its surface, and the specific preparation method is as follows:

[0083] Step 1: Dissolve

[0084] Weigh each material in molar ratio; mix ethylene glycol and distilled water to obtain a mixed system of ethylene glycol and distilled water; wherein, in molar ratio, resorcino...

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Abstract

A hierarchical pore carbon microsphere carrier for immobilized enzymes and its preparation method and application belong to the field of immobilized enzymes. The hierarchical pore carbon microsphere carrier used for immobilizing enzymes has a mesopore-macropore two-stage pore structure, and the mesopore structure exists on the wall of the macropore to form a three-dimensional interconnected pore structure; on the surface and the inner wall of the pore Oxygen-containing functional groups are present; the mesopore diameter is 10‑50 nm and the macropore diameter is 50 <大孔孔径≤220nm，比表面积为306‑631m2 / g. Its preparation method adopts hydrothermal synthesis combined with reverse suspension polymerization method, and then constructs oxygen-containing functional groups on the surface of hierarchical porous carbon microspheres and in the pore wall through active treatment. The preparation method is simple to operate, low in cost, strong in controllability and repeatable. it is good. The immobilization of pectinase by the hierarchical pore carbon microsphere carrier used for immobilizing enzymes of the present invention has the advantages of high activity, good stability, easy recovery and the like.< / 大孔孔径≤220nm，比表面积为306‑631m

Description

technical field [0001] The invention belongs to the technical field of immobilized enzymes, and discloses a hierarchical porous carbon microsphere carrier for immobilized enzymes, a preparation method and application thereof. Background technique [0002] Immobilized enzyme (immobilizedenzyme) is a new technology developed in the 1960s. It is widely used in various fields such as food production, biopharmaceuticals, biosensors, pharmaceutical industry, environmental protection technology and biotechnology. How to improve the activity and stability of immobilized enzymes Reusability, reusability and reproducibility have always been the focus and difficulty of research. Among them, the carrier is an important part of the immobilized enzyme, and its structure and performance have an important impact on the performance and application of the immobilized enzyme. [0003] At present, the carriers used for immobilizing enzymes mainly include inorganic materials, organic materials ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C01B32/05C12N11/14C12N9/88C12N9/26C12N9/18
CPCC01P2006/17C12N9/18C12N9/2408C12N9/88C12N11/14C12Y301/01011C12Y302/01015C12Y402/02002C01B32/05
Inventor 徐昱峰李拖平宋婉莹张佰清张云鹤张红艳石琴展喆
Owner SHENYANG AGRI UNIV
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