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Serum-based brucella vaccine strain infection and wild strain infection rapid identification method

A technology of Brucella and vaccine strains, applied in the field of protein spectrum detection, can solve the problem of not being used to distinguish infection between Brucella vaccine strains and natural wild virus strains.

Active Publication Date: 2020-04-14
ICDC CHINA CDC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, there are no reports at home and abroad on the use of MALDI-TOF MS technology to directly detect serum and confirm Brucel

Method used

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  • Serum-based brucella vaccine strain infection and wild strain infection rapid identification method
  • Serum-based brucella vaccine strain infection and wild strain infection rapid identification method
  • Serum-based brucella vaccine strain infection and wild strain infection rapid identification method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Example 1 Determination of characteristic proteins and construction of standard detection models

[0039] 1. Modeling training sample selection

[0040] The present invention adopts the serum sample of 30 routine Brucella wild strain infection patients, the serum sample of 30 routine Brucella vaccine strain infections, the vaccine strain of Brucella has S2 and A19, all Brucella vaccine strains Infected samples and Brucella wild strain infected samples were confirmed by epidemiological investigation and cysteine ​​agglutination test. 20% of the sample size is used for model cross-validation.

[0041] 2. Enrichment of serum protein samples

[0042] Take out the magnetic bead kit (Bruker MB-WCX kit) from the refrigerator at 4°C, take out a tube of weak cationic magnetic bead suspension, turn it upside down manually, and mix the magnetic bead suspension completely for 1 minute; draw 60 μL of magnetic bead binding buffer ( BB) into a 200 μL sample tube, then add 10 μL of ...

Embodiment 2

[0055] Example 2 Model Classification Ability Test

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Abstract

The invention provides a serum-based rapid identification method for brucella vaccine strain infection and wild strain infection. The method comprises the following steps: acquiring mass spectrometricdata of human serum infected by a brucella vaccine strain and human serum infected by a wild strain; the method comprises the following steps: constructing a standard detection model of serum of a vaccine strain and a wild strain infected with brucella through a supervised neural network (SNN) model algorithm of ClinProTools software; a characteristic protein combination for identifying serum infection of a brucella vaccine strain and a wild strain is obtained, the recognition capability of the constructed model is 100%, and the cross validation value is 95.13%. MALDI-TOF MS mass spectrum data of to-be-detected serum is classified and analyzed by utilizing the standard detection model, so that whether the to-be-detected serum is Brucella vaccine strain infection or wild strain infection or not can be accurately judged. The method is high in accuracy, good in repeatability, high in flux, low in detection cost and reliable in result, and has a good application prospect.

Description

technical field [0001] The invention relates to the technical field of protein spectrum detection, in particular to a standard detection model for detecting serum characteristic proteins and a method for identifying human serum infected with Brucella vaccine strains and wild strains based on the standard detection model. Background technique [0002] Brucellosis (brucellosis, referred to as brucellosis) is an infectious-allergic infectious disease caused by bacteria of the genus Brucella invading the body. Human infection is mainly through contact with infected animals or eating food contaminated by Brucella. After a person suffers from this disease, multiple organs may be damaged, and it is easy to turn into a chronic patient that is difficult to cure. In severe cases, it may become disabled and seriously affect the quality of life. [0003] Animal immunization is the most economical means to prevent and control animal brucellosis, but the antibodies produced after immuniz...

Claims

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Application Information

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IPC IPC(8): G01N27/62G16B5/00G16B50/00
CPCG01N33/6851G01N33/6854G16B5/00G16B50/00G01N2333/23G01N2469/20
Inventor 肖迪姜海张炳华张慧芳王磊杨文涛赵飞李天一
Owner ICDC CHINA CDC
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