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Three-dimensional measurement method for chalkiness of rice based on Micro-CT

A three-dimensional measurement, chalky technology, applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of non-objective, time-consuming, poor consistency and objectivity of traditional methods, and achieve the effect of maintaining biological activity

Pending Publication Date: 2020-04-17
HUNAN AGRICULTURAL UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, traditional methods are less consistent and less objective
This time-consuming and non-objective method is difficult to meet the urgent needs of efficient and accurate determination of rice chalkiness and identification of chalkiness-related phenotypes
In addition, if the chalky volume in the center of the rice is small, it is difficult to observe with the naked eye, which will also cause the measured chalky grain rate to be lower than the actual value.
[0004] Image processing methods have played an important role in the measurement of rice appearance quality, but chalkiness itself is an irregular three-dimensional structure, and the current image processing methods to measure chalkiness are all observed and analyzed from the surface of rice, which is difficult to accurately display Intact chalky interior
In addition, the current image processing method for the measurement of rice chalkiness also requires the process of removing the hulls and grinding the colloidal grains. These processes are often accompanied by the destruction of the embryo, resulting in a complete loss of biological activity.

Method used

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  • Three-dimensional measurement method for chalkiness of rice based on Micro-CT
  • Three-dimensional measurement method for chalkiness of rice based on Micro-CT
  • Three-dimensional measurement method for chalkiness of rice based on Micro-CT

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Embodiment 1

[0025] Embodiment 1 of the present invention is: a kind of rice chalkiness three-dimensional measuring method based on Micro-CT, comprises the following steps:

[0026] Four types of rice chalky rice were collected from different cultivars, i.e. belly white, heart white, whole white and back white (sample photo as figure 2 a) for the volume-based 3D chalkiness quantification experiment. The specific operation steps are as follows:

[0027] 1. Fix and load the rice sample onto a SkyScan 1172CT machine (Bruker, Belgium), which includes a cone-beam X-ray source with a focus less than 5 μm, a sealed cover, distortion correction, air cooling, and a 10MP (pixel) and 12-bit Charge Coupled Device (CCD) camera, coupled by fiber optic Gd 2 o 2 S blinker.

[0028] 2. Place the sample in the sample room, and select the scanning resolution according to the number of samples. The corresponding relationship between the number of rice and the resolution is shown in Table 1 below:

[002...

Embodiment 2

[0040] Embodiment 2 of the present invention is: Micro-CT determination of living rice and its biological activity experiment after scanning, comprising the following steps:

[0041] The rice was fixed and loaded onto a SkyScan 1172CT machine (Bruker, Belgium), which included a cone-beam X-ray source with a focal point smaller than 5 μm, sealed cover, distortion correction, air cooling, and a charge-coupled device with 10MP (pixels) and 12 bits (Charge Coupled Device, CCD) camera, coupled by fiber optic Gd 2 o 2S blinker. Scanning was performed according to the sample size and scanning resolution, the results were exported as DCM format files, and these files were imported into Mimics InnovationSuite Research 19.0 software (Materialise, Belgium) for processing. The rice and chalk are separated by the segmentation tool, and the three-dimensional image is reconstructed by the computational three-dimensional tool to obtain the volume of rice and chalk, and the chalkiness of ric...

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Abstract

The invention discloses a three-dimensional measurement method for chalkiness of rice based on Micro-CT (Micro-Computed Tomography), which comprises the following steps: S1, scanning a rice sample byutilizing a Micro-CT technology to obtain tomography data of rice; and S2, processing and analyzing the acquired data, reconstructing a three-dimensional image, and further calculating the chalkinessdegree of the rice. The processing and analysis process in the step S2 comprises the following steps: carrying out data processing by utilizing Mimics Innovation Suite Research 19.0 software, and reconstructing a three-dimensional image; and after image reconstruction, respectively obtaining the volume of whole rice and the chalkiness, and calculating the chalkiness degree of the rice. The three-dimensional measurement method for chalkiness of rice based on Micro-CT can accurately reflect the chalkiness character, position and volume, can realize three-dimensional measurement of chalkiness ofliving rice, and can obtain a more accurate rice chalkiness measurement value.

Description

technical field [0001] The invention relates to the technical field of rice quality measurement, in particular to a three-dimensional measurement method for rice chalkiness based on MicroCT. Background technique [0002] Rice is one of the main grains in the world. More than half of the world's population and more than two-thirds of China's population use rice as a staple food. Therefore, research on rice yield and quality has attracted worldwide attention. Rice chalkiness is the main factor restricting rice production, because it is one of the key indicators to determine rice quality (appearance, processing, milling, storage, eating and cooking quality) and sales price. Rice chalkiness is light scattering caused by voids caused by poor endosperm filling, and is an opaque part of rice endosperm in appearance that appears white compared to the relatively transparent part. According to the location of the chalkiness in the kernel, the chalky character can be divided into thre...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N23/046
CPCG01N23/046G01N2223/03G01N2223/1016
Inventor 肖浪涛苏益
Owner HUNAN AGRICULTURAL UNIV
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