Hepatitis B surface antigen detection method

Abstract

The invention provides a hepatitis B surface antigen detection method, which comprises the following steps: (1) preparing a serum sample, namely, collecting venous blood, separating the venous blood to obtain serum, and carrying out high-speed centrifugation on the serum 1-2 times to obtain a serum sample to be detected, wherein the high-speed centrifugal conditions are as follows: the temperatureis 4-10 DEG C, the speed is 10000-15000rpm / min, and the time is 3-8min; (2) adding a sample, namely, adding 40-50 [mu] L of the serum sample to be detected into a reaction hole coated with a monoclonal antibody aiming at the hepatitis B surface antigen, wherein the clone number of the monoclonal antibody is 86C; (3) adding an enzyme-labeled antibody, namely, adding 50+ / -1 [mu] L of the enzyme-labeled antibody into the reaction hole, then covering a reaction plate with a plate sealing film, and incubating the reaction plate at 37+ / -1 DEG C for 35-45min; (4) washing; (5) developing; (6) terminating; (7) measuring. The detection method can realize accurate detection of the hepatitis B surface antigen, and particularly has the advantages of smaller sample size required, clear and clean reaction hole body and low background when being used for detecting people with hyperlipidemia.

Description

technical field [0001] The invention belongs to the technical field of molecular detection, in particular to a method for detecting hepatitis B surface antigen. Background technique [0002] Hepatitis B is an infectious disease mainly caused by liver disease caused by hepatitis B virus (HBV). The HBV genome is a double-stranded, circular, incompletely closed DNA consisting of two parts, the outer shell and the core. The outermost layer of the virus is the outer membrane or envelope of the virus, which only contains the virus surface antigen (HBsAg), and the inner layer is the core part (core), and the nucleoprotein is the core antigen (HBcAg). Health physical examination refers to the physical examination of the subject through medical means and methods to understand the health status of the subject, so hepatitis B plays a vital role in the physical examination. At present, the commonly used detection method for hepatitis B virus surface antigen (HBsAg) is enzyme-linked im...

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Problems solved by technology

Obese people and children (children have poor cooperation) are not easy to draw blood, or obese people have high blood viscosity, which leads to a small sample size when drawing blood. During the project, it is easy to cause insufficient serum sample volume, and even need to draw blood again to supplement the sample volume

The inventors of the present invention have also found in practical application that the detected serum samples of obese people (especially those with hyperlipidemia) are less, and it is easy to detect hepatitis B with a variety of conventional ELISA kits. Unclear, Difficult to Judge Questions

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