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Method for rapidly detecting content of scallop adductor muscle glycogen on site

A technology of adductor muscle and glycogen, which is applied in the direction of material analysis by observing the influence of chemical indicators, and analysis by chemical reaction of materials, etc., can solve the problem of inconsistent detection values ​​of glycogen content and cumbersome operation process. and other problems, to achieve the effect of simple and convenient operation and accurate test results

Inactive Publication Date: 2020-04-21
DALIAN OCEAN UNIV
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AI Technical Summary

Problems solved by technology

However, the above-mentioned glycogen content detection methods all need to transport the scallop adductor muscle samples from the field (culture sea area, near shore, food processing workshop and other non-laboratory places) back to the laboratory for determination, and the loss of glycogen during transportation Can cause the detected value of glycogen content to be inconsistent with the actual value on site
Existing detection methods require a variety of precision instruments and equipment such as spectrophotometers and microplate readers during the determination process, and the operation process is cumbersome

Method used

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  • Method for rapidly detecting content of scallop adductor muscle glycogen on site
  • Method for rapidly detecting content of scallop adductor muscle glycogen on site

Examples

Experimental program
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Embodiment Construction

[0012] Take 3 dead Ezo scallops as samples and follow the steps below:

[0013] a. Accurately weigh 1 g of adductor muscle and place it in a transparent glass test tube, add 3 mL of potassium hydroxide solution with a concentration of 30% by mass to the test tube, place the test tube on a self-heating pack and heat for 30 minutes, and after cooling with running water, obtain Adductor muscle lysate;

[0014] b. Take 1 mL of adductor muscle lysis solution and put it in a new transparent test tube, add 19 mL of ultrapure water dropwise, and mix well to obtain glycogen detection solution;

[0015] c. Mix the glycogen detection solution and the glycogen chromogenic solution according to 2.3mL: 2.6mL, and let stand for 20 minutes; the glycogen chromogenic solution is the glycogen chromogenic solution A and the glycogen chromogenic solution B according to 1.3mL : 100mL mixed solution, glycogen chromogenic solution A is a mixed solution of 0.026 g / mL iodine and 0.26 g / mL potassium io...

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Abstract

The invention discloses a method for rapidly detecting a content of scallop adductor muscle glycogen on site, which can realize determination of a glycogen content of adductor muscle food of scallopsthrough simple operations such as glycogen extraction by an alkali liquor, iodine solution color development, colorimetric card comparison and the like on site (culture sea areas, coasts, food processing workshops and other non-laboratory occasions). According to the method, a sample does not need to be transported to a laboratory, precise instruments such as a microplate reader, a spectrophotometer and the like do not need to be used, operation is easy and convenient, rapid detection of the adductor muscle glycogen content can be achieved, and a detection result is accurate.

Description

technical field [0001] The invention relates to a method for detecting the content of glycogen in the adductor muscle of scallops, in particular to a method for quickly detecting the content of glycogen in the adductor muscle of scallops with simple operation and accurate results. Background technique [0002] Scallop is one of the important marine aquaculture economic shellfish in my country, among which adductor muscle is an important food on people's table, and it is also the main raw material for scallop food processing. However, after the scallop dies from water, the glycogen in the adductor muscle will be degraded into fructose-6-phosphate under the action of glycogenolytic enzymes, thus affecting the freshness of the adductor muscle and even causing rancidity. Therefore, glycogen content is an important indicator to determine the freshness of scallop adductor muscle. [0003] At present, the detection methods of glycogen content mainly include colorimetry (anthracene...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/78
CPCG01N21/78
Inventor 宋林生刘超于子超王玲玲
Owner DALIAN OCEAN UNIV
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