Flavones-containing medicine, preparation method thereof, medicine composition and application
A technology of drugs and flavones, which is applied in the field of its preparation and flavone-containing drugs, can solve the problems of difficult to ensure the target group, influence, connection and non-toxic degradation.
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[0153] According to the second aspect of the present application, there is also provided a method for preparing the above-mentioned flavone-containing drug, which includes the following steps: providing any one of the above-mentioned nucleic acid nanoparticles; Loaded on nucleic acid nanoparticles to obtain flavonoid-containing drugs.
[0154] When physically linked, flavonoids usually form intercalations between GC base pairs by physical intercalation. In the case of covalent linkage, flavones usually react with the amino group outside the G ring to form a covalent linkage. The flavonoid-containing drug prepared by the above method can have better targeting after the target head is modified, can stably deliver the flavone, and has high reliability.
[0155] In a preferred embodiment, the step of mounting flavonoids through physical connection includes: mixing and stirring flavones, nucleic acid nanoparticles and the first solvent to obtain a premixed system; precipitating th...
Embodiment 1
[0179] 1. RNA and DNA nanoparticle carriers:
[0180] (1) The base sequences of the three polynucleotides that make up the RNA nanoparticles are shown in Table 1:
[0181] Table 1:
[0182]
[0183] (2) Three polynucleotide base sequences of DNA nanoparticles
[0184] The DNA uses the same sequence as the above RNA, except that T is substituted for U. Among them, the molecular weight of chain a is 8802.66, the molecular weight of chain b is 8280.33, and the molecular weight of chain c is 9605.2.
[0185] The a, b, and c strands of the above-mentioned RNA nanoparticles and DNA nanoparticles were all synthesized by Sangon Bioengineering (Shanghai) Co., Ltd.
[0186] 2. Self-assembly experimental steps:
[0187] (1) RNA or DNA single strands a, b, and c are simultaneously mixed and dissolved in DEPC water or TMS buffer at a molar ratio of 1:1:1;
[0188] (2) Heat the mixed solution to 80°C / 95°C (the RNA assembly temperature is 80°C, and the DNA assembly temperature is 95°C)...
Embodiment 2
[0199] 1. Seven groups of short-sequence RNA nanoparticle carriers:
[0200] (1) The base sequences of the three polynucleotides of the seven groups of RNA nanoparticles are shown in Table 2 to Table 8:
[0201] Table 2: R-1
[0202]
[0203]
[0204] Table 3: R-2
[0205]
[0206] Table 4: R-3
[0207]
[0208] Table 5: R-4
[0209]
[0210]
[0211] Table 6: R-5
[0212]
[0213] Table 7: R-6
[0214]
[0215] Table 8: R-7
[0216]
[0217]
[0218] The single strands of the above seven groups of short-sequence RNA nanoparticle carriers were all synthesized by Sangon Bioengineering (Shanghai) Co., Ltd.
[0219] 2. Self-assembly experimental steps:
[0220] (1) RNA single strands a, b, and c are simultaneously mixed and dissolved in DEPC water or TMS buffer at a molar ratio of 1:1:1;
[0221] (2) Heat the mixed solution to 80°C, keep it for 5min and then cool down slowly to room temperature at a rate of 2°C / min;
[0222] (3) Load the pro...
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