Application of Oxylinalool in Attracting Spodoptera frugiperda and Composition Containing It
A technology for oxidizing linalool and Spodoptera frugiperda, applied in the directions of attracting pests, application, pest control, etc., can solve problems such as ineffective control methods, and achieve the effect of reducing the amount of insects
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Embodiment 1
[0033] Y-tube behavior response
[0034] The experiment was carried out under natural light environment conditions with a room temperature of 24±1°C and a relative humidity of 40±5%. Before the experiment, the Y-shaped olfactometer (Y-shaped tube) was cleaned with acetone and n-hexane successively. Prepare linalool oxide with n-hexane to prepare three concentration gradients of 0.1 μg / μl, 1 μg / μl and 10 μg / μl of the sample to be tested, and use n-hexane as a control, take 10 μl of the sample to be tested and n-hexane to drop in two places respectively. On different pieces of filter paper, put two filter paper strips into the odor source device bottles on both sides of the Y-shaped tube, turn on the atmospheric sampler and make the air flow rate in the Y-shaped tube 100ml / min, and then put the eclosion within 1 day Fall armyworm adults were put into the release tube and observed for 5 minutes. If fall armyworm first selected an arm of more than 5cm and lasted for more than 30s...
Embodiment 2
[0037] Y-tube behavior response
[0038] Oxidized linalool was formulated with n-hexane, natural vegetable oil, dioctyl terephthalate and dichloromethane to prepare a test sample of 1 μg / μl, and each corresponding solvent was used as a control.
[0039] Other operations are the same as in Example 1.
[0040] The behavioral response of the Y-tube results in the following image 3 , the Chi-square test results showed that several solvents dissolved linalool oxide had a significant attractive effect on female insects.
Embodiment 3
[0042] EAG relative response value
[0043] EAG (Syntech, Germany) was used to measure the electrophysiological responses of male and female adults of Spodoptera frugiperda to different concentrations of oxidized linalool. The specific operation steps are as follows: prepare the oxidized linalool with n-hexane to prepare three concentration gradients of 0.1 μg / μl, 1 μg / μl and 10 μg / μl of the samples to be tested, and use n-hexane as a control, and take 10 μl of the samples to be tested each time Or the control drops on the filter paper, and then put the filter paper into the sample tube. The antennae of adults of Spodoptera frugiperda were cut off from the base, and a small part (about 1 mm) of the end of the antennae was cut off, and the two ends of the antennae were respectively glued to the fork-type electrodes with conductive adhesive. Adjust the continuous flow of air at the antenna to 5ml / s. When testing, switch the continuous air flow of air to the stimulating air flo...
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