Rhythmic photostimulation pattern system and application thereof

A light stimulation, rhythmic technology, applied in the field of biomedicine, to achieve the effect of improving osteoporosis, high safety and convenient operation

Inactive Publication Date: 2020-06-02
SHENZHEN INST OF ADVANCED TECH CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] At present, there is no report about the treatment of osteoporosi

Method used

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  • Rhythmic photostimulation pattern system and application thereof
  • Rhythmic photostimulation pattern system and application thereof
  • Rhythmic photostimulation pattern system and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] Construction of a Nude Mouse Model of Osteoporosis Induced by Secondary Hyperparathyroidism

[0046] (1) Gland tissue culture of human secondary hyperparathyroidism:

[0047] Cut the parathyroid tissue from the patient with secondary hyperparathyroidism into 1mm pieces 3 Tissue culture for tissue blocks of different sizes, specifically: place the obtained hyperparathyroid tissue in pre-cooled D-Hanks solution, use fine forceps and surgical scissors to peel off the vascular tissue, and then carefully cut the tissue to 1mm 3 Size: Add culture medium (RPMI1640+10% FBS+1% double antibody) to the 6-well plate, then move the tissue block into it and culture it in a 37°C, 5% CO2 incubator. Subsequent virus infection / transplantation experiments were performed after 3-7 days of culture.

[0048] (2) Virus infection:

[0049] Dilute the lentiviral particles Lenti-CMV-ChETA-eYFP or Lenti-CMV-GFP with RPIM1640 medium without serum and double antibody at a concentration of 1:200 ...

Embodiment 2

[0053] Photostimulation test:

[0054] One week after the operation of the nude mice described in Example 1, the transplanted parathyroid glands were stimulated by blue light for four weeks, which were divided into two modes: (1) stimulated three times a week, each time for 30 minutes, and the light wavelength was 470nm , light frequency 20Hz, 50ms stimulation interval; (2) stimulation three times a week, each containing two 15min (15min stimulation in the morning, 15min stimulation in the afternoon), light wavelength 470nm, light frequency 20Hz, 50ms stimulation interval.

Embodiment 3

[0056] In this example, kits (PTH measurement: Mouse PTH ELISA Kit, LSBio, product number LS-F5549; blood calcium determination: Calcium Colormetric Assay sufficient for 250colorimetric tests, Sigma, product number MAK022) were used to test the nude mice in each group after the operation in Example 2. The changes of parathyroid hormone and blood calcium concentration in serum were monitored (for mode (1), blood samples were collected before stimulation, immediately after stimulation, 5min after stimulation, 15min after stimulation, and 30min after stimulation for PTH and blood Calcium analysis; for mode (2), blood samples were also collected before stimulation, immediately after stimulation, 5min after stimulation, 15min after stimulation, and 30min after stimulation for PTH and blood calcium analysis. The purpose is to compare and analyze the effects of different stimulation modes on Effects of PTH and Calcium).

[0057] The result is as Figure 1-Figure 6 shown (where figu...

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Abstract

The invention relates to a rhythmic photostimulation pattern system and application thereof. In the rhythmic photostimulation pattern system, a cycle of photostimulation is four weeks, the frequency of photostimulation is three times per week, and the time of photostimulation at a time is 10 to 40 minutes. The rhythmic photostimulation pattern system disclosed by the invention can be used for effectively improving osteoporosis induced by secondary hyperparathyroidism, remarkably inhibiting excessive secretion of parathyroid hormones induced by the secondary hyperparathyroidism, remarkably reversing bone density and/or bone bulk density decrease induced by the secondary hyperparathyroidism, activating differentiating and aging of osteoblasts and inhibiting differentiating and aging of osteoclasts, and thus, osteogenesis can be greater than osteoclastogenesis. The rhythmic photostimulation pattern system is very convenient in operating mode, and an entire process is free of introductionof drug for intervention, so that a side effect resulting from employing the system is lower compared with that of drug therapy, and the safety is higher.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, and in particular relates to a rhythmic light stimulation mode system and its application. Background technique [0002] Parathyroid hormone (PTH) is secreted by parathyroid chief cells, an important calcium and phosphorus homeostasis regulator composed of 84 amino acids, which can regulate its own expression and secretion according to changes in blood calcium concentration. It plays an important role in maintaining the body's blood calcium and phosphorus balance and bone metabolism balance. Intermittent administration of low doses of PTH can promote the maturation and differentiation of osteoblasts, mediate the process of osteogenesis, and increase bone mass; while continuous administration of high doses of PTH can promote the maturation of osteoclasts, thereby mediating the process of osteoclasts and inducing osteoclasts. Bone loss. PTH is clinically used to treat osteoporosis in postmeno...

Claims

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Application Information

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IPC IPC(8): A61N5/06
CPCA61N5/0613A61N2005/0627
Inventor 杨帆刘运辉张路
Owner SHENZHEN INST OF ADVANCED TECH CHINESE ACAD OF SCI
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