Method for determining xylanase added into feed

A technology of xylanase and determination method, which is applied in the field of feed, can solve the problems of large coefficient of variation of detection results and inability to measure accurately, and achieve the effect of reducing the lower limit of detection, eliminating the influence of interference background, and avoiding influence

Pending Publication Date: 2020-06-12
GUANGDONG VTR BIO TECH
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

[0006] Using GB / T23874-2009 to measure xylanase from different sources, the coefficient of variation of the test results is large and cannot be accurately determined. Therefore, a fast, simple, highly specific, sensitive, and accurate method is needed to determine xylanase. The activity of carbohydrase and the activity of xylanase added in the feed will be more conducive to the scientific and rational formulation and promote the development of xylanase industry

Method used

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  • Method for determining xylanase added into feed
  • Method for determining xylanase added into feed
  • Method for determining xylanase added into feed

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] 1. Measurement steps

[0046] 1.1 Standard curve

[0047] Accurately weigh a certain amount of standard xylanase (refer to the national standard GB / T 23874-2009 for the limitation of labeled feed xylanase), accurate to 0.0001g, dissolve it in a 50mL volumetric flask with acetic acid buffer and determine Make up to the mark, make two dilutions, so that the xylanase activity is about 0.20U / mL, and prepare on the same day.

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Abstract

The invention relates to the field of feeds, in particular to a method for determining xylanase added into a feed. The method comprises the following steps: pre-cooling a to-be-detected feed sample, dialyzing with an acetic acid buffer solution, and determining the enzyme activity. According to the trace determination method of the xylanase added into the feed, through pretreatment of the feed sample, inactivation of the xylanase in the extraction process is avoided, the lower limit of xylanase detection is reduced, and influence of background interference is eliminated, so that the content ofthe xylanase added into the feed can be accurately determined.

Description

technical field [0001] The invention relates to the field of feed, in particular to a method for measuring xylanase added in feed. Background technique [0002] In 2009, the national standard GB / T 23874-2009 for the determination method of feed xylanase activity was released. One unit of xylanase activity is defined as: at 37°C and pH 5.5 from a concentration of 5mg per minute The amount of xylanase required to release 1 μmol of reducing sugar per mL of xylan solution. [0003] In the existing determination method (GB / T23874-2009), the principle of "DNS method" to determine the content of xylanase is: under certain temperature and pH conditions, xylanase hydrolyzes xylan to generate oligosaccharides and monosaccharides , Reducing oligosaccharides and monosaccharides can undergo a color reaction with 3,5-dinitrosalicylic acid (DNS) reagent in a boiling water bath, and perform colorimetric determination at a wavelength of 540nm. The specific process is: take 2.0mL of the rea...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/34
CPCC12Q1/34G01N2333/924
Inventor 崔细鹏彭宇邹嘉豪李阳源柴毛毛周银华
Owner GUANGDONG VTR BIO TECH
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