Human-adipose derived adult stem cell-derived neuronal precursor cells and preparation method therefor and application of neuronal precursor cells

An adipose stem cell and precursor cell technology, applied in biochemical equipment and methods, animal cells, nervous system cells, etc., can solve problems such as low yield, purity and activity, poor neuron-like cell health, etc. Better behavior and better health

Pending Publication Date: 2020-06-19
上海泉眼生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] In order to solve the problems of poor health, low yield, purity and activity of neuron-like cells induced by existing adipose-derived stem cells, the object of the pres...

Method used

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  • Human-adipose derived adult stem cell-derived neuronal precursor cells and preparation method therefor and application of neuronal precursor cells
  • Human-adipose derived adult stem cell-derived neuronal precursor cells and preparation method therefor and application of neuronal precursor cells
  • Human-adipose derived adult stem cell-derived neuronal precursor cells and preparation method therefor and application of neuronal precursor cells

Examples

Experimental program
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Embodiment 1

[0038] The preparation process of the induction medium is as follows:

[0039] Add NEAA, β-mercaptoethanol, glutamine, growth factors and neurotrophic factors to the basal medium, wherein the volume percentage of NEAA is 1%, the volume percentage of β-mercaptoethanol is 0.15%, and the volume percentage of glutamine The growth factor is composed of 10ng / mL bFGF and 20ng / mL EGF, and the neurotrophic factor is composed of 20ng / mL BDNF, 10ng / mL NGF and 10ng / mL NT3.

Embodiment 2

[0041] The preparation process of the induction medium is as follows:

[0042] Add NEAA, β-mercaptoethanol, glutamine, growth factors and neurotrophic factors to the basal medium, wherein the volume percentage of NEAA is 2%, the volume percentage of β-mercaptoethanol is 0.25%, and the volume percentage of glutamine The growth factor is composed of 20ng / mL bFGF and 10ng / mL EGF, and the neurotrophic factor is composed of 10ng / mL BDNF, 20ng / mL NGF and 20ng / mL NT3.

Embodiment 3

[0044] The preparation process of the induction medium is as follows:

[0045] Add NEAA, β-mercaptoethanol, glutamine, growth factors and neurotrophic factors to the basal medium, wherein the volume percentage of NEAA is 1.5%, the volume percentage of β-mercaptoethanol is 0.2%, and the volume percentage of glutamine The growth factor is composed of 15ng / mL bFGF and 15ng / mL EGF, and the neurotrophic factor is composed of 15ng / mL BDNF, 15ng / mL NGF and 15ng / mL NT3.

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Abstract

The invention discloses an induction medium. The medium is employed to perform induction culture on a human-adipose derived adult stem cell to make the human-adipose derived adult stem cell differentiated into neuronal precursor cells, and comprises non-essential amino acid (NEAA), beta-mercaptoethanol, glutamine, a growth factor and a neurotrophic factor; the volume ratio of the NEAA to the beta-mercaptoethanol is (4:1)-(13:1); the volume ratio of the NEAA to the glutamine is (1:2)-(2:1); the growth factor consists of a basic fibroblast growth factor (bFGF) of 10-20 ng/mL and an epidermal growth factor (EGF) of 10-20 ng/mL; and the neurotrophic factor consists of a brain derived neurotrophic factor (BDNF) of 10-20 ng/mL, a nerve growth factor (NGF) of 10-20 ng/mL and neurotrophin-3 (NT3)of 10-20 ng/mL. A human-adipose derived adult stem cell-derived neuronal precursor cell population disclosed by the invention is obtained by inducing the human-adipose derived adult stem cells to be differentiated by the specific induction medium, and the neuronal precursor cells have a good health status and are high in activity and purity.

Description

technical field [0001] The invention relates to the technical field of stem cells and biomedicine, in particular to neuron precursor cells derived from human adipose stem cells, a preparation method and its application in treating nervous system diseases. Background technique [0002] Human-Adipose Derived Adult Stemcell (hADSC) has become the most promising cell due to its advantages of easy acquisition, low aging rate in vitro culture, low immunogenicity, high biological safety, and no ethical issues involved. one of the sources. Differentiation of adipose-derived stem cells into nerves is a key step in their clinical transformation to treat neurological diseases. At present, there are compound methods and gene reprogramming methods for inducing adipose-derived stem cells to differentiate into nerves. The disadvantage of the compound method is that the compound has toxic side effects on the cells, which affects the health of the differentiated neurons. At the same time, ...

Claims

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Application Information

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IPC IPC(8): C12N5/0797C12N5/0793A61K35/30A61P25/00A61P25/28A61P25/16
CPCC12N5/0619A61K35/30A61P25/00A61P25/28A61P25/16C12N2506/1384C12N2500/44C12N2500/32C12N2501/13C12N2501/115C12N2501/11
Inventor 高山峨
Owner 上海泉眼生物科技有限公司
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