The invention discloses a large amount attachment and culture method of monostroma zygote. The method comprises the steps of S1, collecting monostroma as seed algae, and drying the monostroma in shade for 12-14 hours indoors; S2, adding the seed algae into sea water for culturing, inflating and stirring the seed algae in strong illumination, adding distilled water at the same time, and conducting filtration after 15-25 min to obtain a gamete solution; S3, placing seedling plates in the gamete solution obtained in S2, conducting treatment in darkness for 9-12 hours, conducting zygote attachment, conducting intermittent illumination culture at certain temperature, conducting zygote culturing, keeping the illumination cycle of 12 hours per day, replacing half of the seawater every 15 days, and conducting culturing for 5-7 months. According to the method for large-amount attachment and culture of monostroma zygote on the seedling plates, a large amount of high purity monostroma gamete solution can be stably obtained at one time, and the attachment and culture of the zygote can be carried out. The method provides the technical foundation for the large-scale artificial breeding and culture of monostroma, and possesses a promotion and application prospect in the obtainment and culturing of green algae zygote.