Large amount attachment and culture method of monostroma zygote

Abstract

The invention discloses a large amount attachment and culture method of monostroma zygote. The method comprises the steps of S1, collecting monostroma as seed algae, and drying the monostroma in shade for 12-14 hours indoors; S2, adding the seed algae into sea water for culturing, inflating and stirring the seed algae in strong illumination, adding distilled water at the same time, and conducting filtration after 15-25 min to obtain a gamete solution; S3, placing seedling plates in the gamete solution obtained in S2, conducting treatment in darkness for 9-12 hours, conducting zygote attachment, conducting intermittent illumination culture at certain temperature, conducting zygote culturing, keeping the illumination cycle of 12 hours per day, replacing half of the seawater every 15 days, and conducting culturing for 5-7 months. According to the method for large-amount attachment and culture of monostroma zygote on the seedling plates, a large amount of high purity monostroma gamete solution can be stably obtained at one time, and the attachment and culture of the zygote can be carried out. The method provides the technical foundation for the large-scale artificial breeding and culture of monostroma, and possesses a promotion and application prospect in the obtainment and culturing of green algae zygote.

Description

technical field [0001] The invention belongs to the technical field of algae cultivation, and more specifically relates to a method for mass attachment and cultivation of reef membrane zygotes. Background technique [0002] Reef film is a kind of marine economical green algae widely distributed, rich in nutrition, high in protein, carbohydrates, vitamins and mineral elements, good taste, high edible value, and medicinal value, can clear away heat and reduce phlegm , diuresis and detoxification, softening and resolving stagnation, can be used to treat laryngitis, cough and phlegm, edema, etc. The sulfated polysaccharide extracted from it has anti-coagulation, anti-oxidation, blood lipid-lowering, and anti-inflammatory activities. It is a very potential development Sea food, drug and cosmetic resources. [0003] Reef film has already entered commercial cultivation in Japan and South Korea for people to eat. In terms of output value and cultivation scale, it is second only to ...

AI Technical Summary

Problems solved by technology

[0005] At present, the method of raising seedlings on reef film is mainly to attach seedlings with net curtains in wild reef film production areas in good time, but the efficiency of this method is low and unstable, the yield is not high, and there are too many miscellaneous algae; at present, although wide reef film, The artificial seedling experiment of arctic reef membrane, but in the prior art, there is no discussion on the method of a large number of attached zygotes and culture at one time. They are all just a small amount of observation experiments, which are far from meeting the needs of a large number of attached zygotes at one time in the process of artificial seedling cultivation.

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