A nanoprobe for CT imaging of Kawasaki disease coronary artery disease and its preparation method and application

A nanoprobe and CT imaging technology, which is applied in the field of nanoprobe and its preparation for CT imaging of Kawasaki disease coronary artery disease, can solve the problem of unrecognizable vascular-related lesions, achieve clear pharmacological and toxicological processes in vivo, and improve probe Potency, the effect of prolonging the existence time

Active Publication Date: 2022-02-25
FOURTH MILITARY MEDICAL UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, these examinations can often only diagnose the aneurysm that has formed, but cannot identify the vascular-related lesions before the formation of the aneurysm
However, there is currently no report on OPN as a nanoprobe targeting coronary vascular lesions in Kawasaki disease

Method used

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  • A nanoprobe for CT imaging of Kawasaki disease coronary artery disease and its preparation method and application
  • A nanoprobe for CT imaging of Kawasaki disease coronary artery disease and its preparation method and application
  • A nanoprobe for CT imaging of Kawasaki disease coronary artery disease and its preparation method and application

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Experimental program
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Embodiment 1

[0039] (1) Weigh 192mL H with a 250mL round-bottom three-neck flask 2 O, add 2 mL of 1% HAuCl 4 , stirred at 650 rpm, heated to boil, condensed to reflux, added 6 mL of 1% sodium citrate, and continued to stir for 15 min.

[0040] (2) 160 mg SH-PEG-COOH was added to 200 mL of 15 nm Au nanoparticles, fully stirred, and left to react overnight at room temperature, so that the surface of the Au nanoparticles had carboxyl functional groups. The 15nm Au nanoparticles modified with SH-PEG-COOH were concentrated and purified by ultrafiltration with a 100KD ultrafiltration tube, and the final volume was adjusted to 10 mL, so that the concentration of Au nanoparticles was 1 mg / mL, and sterilized through a 0.22 μm filter membrane.

[0041] (3) Take 4 mL of SH-PEG-COOH-conjugated 15 nm Au nanoparticles (1 mg / mL), add 4 mL of 100 mM MES, pH=4.8, add 1 mg of OPN antibody (30 mg / mL, 33 μL), and absorb at 37°C for 1 h on a shaker , add EDC 240mg / mL, 160μL, shake overnight at 37°C on a shak...

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Abstract

The invention discloses a nanoprobe for CT imaging of Kawasaki disease coronary artery disease and its preparation method and application. The nanoprobe is an osteopontin-targeted nanoprobe anti-OPN-PEG-Au, which is composed of Osteopontin antibody is condensed with SH-PEG-COOH-modified gold nanoparticles, and the active amino group of osteopontin antibody is condensed with the active carboxyl group on the surface of SH-PEG-COOH-modified gold nanoparticles. The nanoprobe produced by the preparation method of the present invention can realize in vivo imaging of Kawasaki disease coronary artery inflammation, and thus has a good application prospect in early warning of coronary aneurysms. Simultaneously, the preparation is simple, and industrial production is easy to realize.

Description

technical field [0001] The invention belongs to the technical field of medical diagnostic reagents, and relates to a molecular probe in a medical diagnostic reagent and a preparation method thereof, in particular to a nano-probe used for CT imaging of Kawasaki disease coronary arteritis, and a preparation method and application thereof. Background technique [0002] Kawasaki disease (KD), which occurs in children from 6 months to under 5 years old, is a serious and far-reaching vascular inflammatory disease. It mainly invades the vascular walls of medium and small arteries, especially the coronary arteries, and has become the main cardiovascular disease that threatens the lives of children today. Among the many cardiovascular complications caused by Kawasaki disease, the incidence and mortality of coronary artery damage events occupy the first place, and among them, coronary artery aneurysm has the most serious consequences. Studies have shown that without timely treatment,...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K49/04B82Y25/00
CPCA61K49/04B82Y25/00
Inventor 乔红玉张若涵
Owner FOURTH MILITARY MEDICAL UNIVERSITY
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