Nanoprobe for CT imaging of Kawasaki disease coronary artery inflammation, and preparation method and application thereof

A nanoprobe and CT imaging technology, applied in the field of nanoprobe and its preparation for CT imaging of Kawasaki disease coronary artery disease, can solve the problem of unrecognizable vascular-related lesions, achieve clear pharmacological and toxicological processes in vivo, and prolong the existence time , Improve the effect of probe performance

Active Publication Date: 2020-06-23
FOURTH MILITARY MEDICAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, these examinations can often only diagnose the aneurysm that has formed, but cannot identify the vascular-related lesions before the formation of the aneurysm
However, there is currently no report on OPN as a nanoprobe targeting coronary vascular lesions in Kawasaki disease

Method used

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  • Nanoprobe for CT imaging of Kawasaki disease coronary artery inflammation, and preparation method and application thereof
  • Nanoprobe for CT imaging of Kawasaki disease coronary artery inflammation, and preparation method and application thereof
  • Nanoprobe for CT imaging of Kawasaki disease coronary artery inflammation, and preparation method and application thereof

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Embodiment 1

[0039] (1) Weigh 192mL of H in a 250mL round-bottom three-neck flask 2 O, add 2 mL of 1% HAuCl 4 , stirred at 650rpm, heated to boil, condensed to reflux, added 6mL of 1% sodium citrate, and continued to stir and react for 15min.

[0040] (2) Add 160mg of SH-PEG-COOH to 200mL of 15nm Au nanoparticles, stir well, and leave to react at room temperature overnight, so that the surface of the Au nanoparticles has carboxyl functional groups. The 15nm Au nanoparticles modified with SH-PEG-COOH were concentrated and purified by ultrafiltration with a 100KD ultrafiltration tube, and finally fixed to 10mL so that the concentration of Au nanoparticles was 1mg / mL, and sterilized by a 0.22μm filter membrane.

[0041] (3) Add 4mL of 15nm Au nanoparticles (1mg / mL) coupled with SH-PEG-COOH to 4mL of 100mM MES, pH=4.8, add 1mg of OPN antibody (30mg / mL, 33μL), and absorb on a shaker at 37°C for 1h , add EDC 240mg / mL, 160μL, and shake overnight at 37°C on a shaker. The 15nm Au nanoparticles r...

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Abstract

The invention discloses a nanoprobe for CT imaging of Kawasaki disease coronary artery inflammation, and a preparation method and application thereof. The nanoprobe is an osteopontin targeted nanoprobe anti-OPN-PEG-Au, and is formed by osteopontin antibodies and SH-PEG-COOH modified nanogold particles through condensation; and the active amino groups of the osteopontin antibodies and the active carboxyl groups on the surfaces of the SH-PEG-COOH modified nanogold particles are connected through condensation. Through the nanoprobe prepared by the preparation method provided by the invention, thein vivo imaging of the Kawasaki disease coronary artery inflammation can be realized, so that good application prospects are realized in the aspect of early warning of coronary aneurysm. Meanwhile, the preparation is simple, and the industrial production can be easily realized.

Description

technical field [0001] The invention belongs to the technical field of medical diagnostic reagents, and relates to a molecular probe in a medical diagnostic reagent and a preparation method thereof, in particular to a nanoprobe for CT imaging of Kawasaki disease coronary artery disease, a preparation method and application thereof. Background technique [0002] Kawasaki disease (KD) is a vascular inflammatory disease with great harm and far-reaching effects, which occurs in children from 6 months to less than 5 years old. It mainly invades the vessel walls of medium and small arteries, especially the coronary arteries, and has become the main cardiovascular disease that threatens the lives of children today. Among the many cardiovascular complications caused by Kawasaki disease, the incidence and fatality rate of coronary artery damage events occupy the top of the list, and among them, the consequences caused by coronary aneurysms are the most serious. Studies have shown th...

Claims

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Application Information

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IPC IPC(8): A61K49/04B82Y25/00
CPCA61K49/04B82Y25/00
Inventor 乔红玉张若涵
Owner FOURTH MILITARY MEDICAL UNIVERSITY
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