Healthy aging serum protein marker and application

A serum protein and marker technology, applied in the field of biomedicine, to achieve high accuracy and high sensitivity

Inactive Publication Date: 2020-06-26
KUNMING INST OF ZOOLOGY CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, there are no domestic and international reports on the above-mentioned markers that can indicate healthy aging in real time

Method used

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  • Healthy aging serum protein marker and application
  • Healthy aging serum protein marker and application
  • Healthy aging serum protein marker and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Example 1 Healthy Senescence Protein Marker

[0038] Through a prospective study on the serum proteome of 119 long-lived elderly (95-111 years old, median 100 years old) and 151 elderly controls (32-80 years old, median 61 years old) in Hainan Province, the present study The invention provides a serum protein marker of healthy aging, which can be used alone or in combination of two or more to carry out molecular assessment of healthy aging, including apolipoprotein L1 (Apolipoprotein L1, APOL1), insulin-like growth Factor binding protein 3 (Insulin-like growth factor-binding protein 3, IGFBP3), insulin-like growth factor-binding protein complex acid labile subunit (Insulin-like growth factor-binding protein complex acid labile subunit, IGFALS), vitronectin (Vitronectin, VTN), plasminogen (Plasminogen, PLG), fibrillin-1 (Fibrillin-1, FBN1), macrophage mannose receptor 1 (Macrophage mannose receptor1, MRC1), complement factor D (Complement factor D, CFD), EGF-containing ...

Embodiment 2

[0045] Example 2 Assessment of healthy aging with a single marker such as apolipoprotein L1:

[0046] The specific implementation method is as follows:

[0047] 1. Professional medical staff draw blood from at least 3 subjects (at least 3 samples are required to calculate the average protein abundance), obtain serum samples by centrifugation, and store them at -80°C;

[0048] 2. Measuring the expression abundance of apolipoprotein L1 in serum samples. For example, the relative quantitative method based on the mass spectrometry platform (Q-Exactive) - TMT, to obtain the expression abundance of apolipoprotein L1, the detailed steps are as follows:

[0049] a). Protein extraction: Serum samples were taken out from -80°C, centrifuged at 12,000g for 10 minutes at 4°C to remove cell debris, and the supernatant was transferred to a new centrifuge tube. The kit produced by Thermo was referred to Pierce TM Top 12AbundantProtein Depletion Spin Columns Kit instructions to remove high-a...

Embodiment 3

[0056] Example 3 Evaluation of healthy aging with a single marker such as leucine-rich α2 glycoprotein:

[0057] Directly design a kit or test paper for measuring the abundance of leucine-rich α2 glycoprotein, and use the kit or test paper to measure the abundance of leucine-rich α2 glycoprotein in a small amount of blood of the subject (similar to a blood glucose meter that directly measures blood sugar through a drop of blood) Abundance mode) 3 individuals were randomly selected from the 151 elderly groups mentioned in Example 1: subject a, subject b, subject c, and their relative protein expression values ​​were: 3.662 2.796 1.706 . The average protein expression value of all subjects was calculated as 2.721, which was used as the reference level of leucine-rich α2 glycoprotein in this group of subjects. For a single subject, such as subject a, the expression level of leucine-rich α2 glycoprotein is 3.662 higher than 2.721. Looking up Table 1, it can be seen that healthy a...

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Abstract

The invention discloses a healthy aging serum protein marker and application thereof. The marker comprises 19 proteins. According to the invention, the protein markers for health aging are extracted from the serum protein level for the first time, and have the characteristics of high accuracy and high sensitivity (AUC is greater than 0.800, and the highest sensitivity is 0.878). The method can bedirectly used as an evaluation index of health aging.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, and in particular relates to a healthy aging serum protein marker and its application. Background technique [0002] The aging rate of my country's population is more than twice that of western countries, among which the elderly over 65 years old will increase from 119 million in 2010 to 360 million in 2050 (accounting for 25.6% of the total population). Generally, the elderly population over the age of 65, with the increase of age, the incidence index of aging-related diseases increases, which eventually leads to their death due to aging diseases. But among the naturally aging population, there is a special group of people—the long-lived elderly: they rarely suffer from aging-related diseases on the basis of being able to live to the age of 90 or more. Compared with the general aging population, the aging pattern of the long-lived elderly is called "healthy aging". The intensification of t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68G01N33/573
CPCG01N33/573G01N33/6893G01N2800/60
Inventor 孔庆鹏尹藩乾李功华
Owner KUNMING INST OF ZOOLOGY CHINESE ACAD OF SCI
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