Gene, expression vector and application for inhibiting proliferation, invasion and metastasis of breast cancer cells

Abstract

The invention belongs to the technical field of genetic engineering, and discloses a gene, expression vector and application for inhibiting proliferation, invasion and metastasis of breast cancer cells. The gene is LINC00850, which is a long-chain non-coding RNA that does not have the ability to encode proteins, and the length is greater than 200nt ;LINC00850 is located on the X chromosome, the length is 697bps, and the sequence name is NR_109813. The present invention detects the expression of LINC00850 in normal breast epithelial cells MCF-10A and breast cancer cells; compared with normal breast epithelial cells MCF-10A, the expression of LINC00850 in breast cancer cells is significantly up-regulated, and in highly invasive cells MDA-10A Expression was highest in MB‑231 and lowest in the less invasive cell MCF‑7. The invention has important significance for the diagnosis and treatment of breast cancer.

Description

technical field [0001] The invention belongs to the technical field of genetic engineering, and in particular relates to a gene, expression carrier and application for inhibiting the proliferation, invasion and metastasis of breast cancer cells. Background technique [0002] At present, the closest existing technology: female breasts are composed of skin, fibrous tissue, mammary glands and fat, and breast cancer is a malignant tumor that occurs in the mammary gland epithelial tissue. 99% of breast cancers occur in women and only 1% in men. The mammary gland is not an important organ to maintain human life activities, and breast cancer in situ is not fatal; but because breast cancer cells lose the characteristics of normal cells, the connections between cells are loose and easy to fall off. Once the cancer cells fall off, the free cancer cells can spread throughout the body with the blood or lymph fluid, forming metastasis, which is life-threatening. At present, breast canc...

AI Technical Summary

Problems solved by technology

[0004] (1) In the prior art, no theoretical basis can be provided for the expression in breast cancer cells, the expression in the highly invasive cell MDA-MB-231, and the expression in the low invasive cell MCF-7

[0005] (2) The existing technology cannot accurately inhibit the proliferation, invasion and metastasis of breast cancer

The clinical treatment of breast cancer cannot achieve the effect of inhibiting proliferation, invasion and metastasis, and cannot improve the survival rate of patients

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