Compound microbial bacterial preparation and microecological preparation for sewage treatment and applications
A technology of compound microbial bacterial agent and microecological preparation, which is applied in the direction of biological water/sewage treatment, microbial-based methods, water/sludge/sewage treatment, etc. It can solve the problems of easy pollution, poor self-purification ability of water body, and lack of oxygen in water body, etc. problem, to achieve good application prospects, reduce the intensity of odor, and increase the effect of transparency
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Embodiment 1
[0043] Example 1. Preparation of compound microbial bacterial agent and functional detection of nitrogen and phosphorus removal
[0044] 1 Preparation of single bacterial agent and compound microbial bacterial agent
[0045] 1.1 Preparation of cellulobacteria DSM 16025 single agent
[0046] Cellulobacterium DSM 16025 was inoculated in liquid LB medium, 30 DEG C, 150rpm shaking culture 24h, obtain cellulobacteria DSM 16025 bacterial liquid, the content of cellulobacterium DSM 16025 in this cellulobacterial DSM 16025 bacterial liquid was 2 ×10 8 cfu / ml, the cellulobacterium DSM 16025 bacterial solution is the cellulobacterium DSM 16025 single bacterial agent.
[0047] 1.2 Preparation of denitrifying Zobelella DSM 19707 single bacteria agent
[0048] Inoculate the denitrifying Zobelella DSM 19707 in the liquid LB medium, shake and culture at 30°C and 150rpm for 24 hours to obtain the denitrifying Zobella DSM 19707 bacterial liquid, and the denitrifying Zobelella DSM 19707 bact...
Embodiment 2
[0098] Embodiment 2, detection of compound microbial bacterial agent black and odorous water treatment function
[0099] 1 Preparation of single bacterial agent and compound microbial bacterial agent
[0100] 1.1 Preparation of cellulosic bacteria DSM16025 single bacterial agent
[0101] Cellulobacteria DSM16025 was inoculated in liquid LB culture medium, 30 DEG C, 150rpm shaking culture 24h, obtain cellulobacterium DSM16025 bacterium liquid, the content of the cellulobacterium DSM16025 in this cellulobacterium DSM16025 bacterium liquid is 2 * 10 8 cfu / ml, the cellulobacterium DSM16025 bacterial liquid is the cellulobacterium DSM16025 single bacterial agent.
[0102] 1.2 Preparation of denitrifying Zobelella DSM19707 single bacterial agent
[0103] Inoculate the denitrifying Zobelella DSM19707 in liquid LB medium, culture at 30°C and 150rpm for 24 hours with shaking, and obtain the denitrifying Zobella DSM19707 bacterial liquid, the denitrifying Zobelella DSM19707 bacterial ...
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