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Immune lotion for electrochemical luminescence immunoassay analyzer

A luminescence immunoassay, analyzer technology, applied in chemiluminescence/bioluminescence, analysis by chemical reaction of materials, analysis of materials, etc., can solve the problem of large amount of buffer and cleaning solution, increase the cost of detection, and large consumption and other problems to achieve the effect of increasing the cleaning effect, reducing the cost and reducing the dosage

Active Publication Date: 2020-08-14
长春晨裕生物医疗科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Its electrochemiluminescence system is terpyridine ruthenium-tripropylamine system, the amount of buffer and cleaning solution used in supporting it is relatively large, and the matching original reagents, especially the buffer, are expensive and highly toxic
[0004] At present, the major hospitals in our country generally use the original cleaning solution, and the consumption is huge
It is expensive, which greatly increases the cost of detection
Although there are many technologies disclosed in the prior art, such as patent 201710877908.4, although the cost is reduced, tripropylamine is a toxic substance and the dosage is too large; although the patent 201610538476.X reduces the dosage of tripropylamine, the MES used is also toxic. Therefore, in theory, the toxicity of the cleaning solution prepared by it has not been reduced

Method used

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  • Immune lotion for electrochemical luminescence immunoassay analyzer
  • Immune lotion for electrochemical luminescence immunoassay analyzer

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] 1) The components and contents of the buffer are: Potassium dihydrogen phosphate 200mmol / L, tripropylamine 3mmol / L, N,N-dibutylethanolamine 1mmol / L, potassium chloride 5mmol / L, polidocanol 5mmol / L L, polyoxyethylene alkanolamide 3mmol / L, hydroxypropyl methylcellulose 0.5mmol / L, Proclin 300 0.1% (w%);

[0024] Preparation of buffer solution: Weigh the components except tripropylamine and add to a small amount of water to stir to dissolve, add tripropylamine and stir to stand until completely dissolved, then transfer to a volumetric flask, constant volume, shake well, add sodium hydroxide Adjust the pH, pH 6.5, that is.

[0025] 2) The composition and content of the cleaning solution are: potassium hydroxide 160mmol / L, alkylphenol polyoxyethylene ether 0.1% (w%), dodecyl dextrose 0.5% (w%), Pluronic L81 1.5% (w%), Proclin300 0.1% (w%).

[0026] Preparation of cleaning solution: Weigh each raw material according to the ingredients in the above formula, add water to disso...

Embodiment 2

[0028] 1) The components and contents of the buffer are: potassium dihydrogen phosphate 300mmol / L, tripropylamine 8mmol / L, N,N-dibutylethanolamine 3mmol / L, potassium chloride 8mmol / L, polydocanol 8mmol / L L, polyoxyethylene alkanolamide 5mmol / L, hydroxypropyl methylcellulose 0.8mmol / L, Proclin 300 0.5% (w%);

[0029] Preparation of buffer solution: Weigh the components except tripropylamine and add to a small amount of water to stir to dissolve, add tripropylamine and stir to stand until completely dissolved, then transfer to a volumetric flask, constant volume, shake well, add sodium hydroxide Adjust the pH, the pH value is 7.3, that is.

[0030] 2) The composition and content of the cleaning solution are: potassium hydroxide 190mmol / L, alkylphenol polyoxyethylene ether 1% (w%), dodecyl dextrose 1% (w%), Pluronic L81 5% (w%), Proclin 3000.5% (w%).

[0031] Preparation of cleaning solution: Weigh each raw material according to the ingredients in the above formula, add water t...

Embodiment 3

[0033] 1) The components and contents of the buffer are: Potassium dihydrogen phosphate 250mmol / L, tripropylamine 4mmol / L, N,N-dibutylethanolamine 2mmol / L, potassium chloride 5mmol / L, polydocanol 8mmol / L L, polyoxyethylene alkanolamide 4mmol / L, hydroxypropyl methylcellulose 0.6mmol / L, Proclin 300 0.2% (w%);

[0034] Preparation of buffer solution: Weigh the components except tripropylamine and add to a small amount of water to stir to dissolve, add tripropylamine and stir to stand until completely dissolved, then transfer to a volumetric flask, constant volume, shake well, add sodium hydroxide Adjust the pH, pH 6.8, that is.

[0035] 2) The composition and content of the cleaning solution are: potassium hydroxide 180mmol / L, alkylphenol polyoxyethylene ether 0.5% (w%), dodecyl dextrose 0.7% (w%), Pluronic L81 3% (w%), Proclin300 0.3% (w%).

[0036] Preparation of cleaning solution: Weigh each raw material according to the ingredients in the above formula, add water to dissolv...

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PUM

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Abstract

The invention belongs to the field of medical instrument in-vitro diagnostic reagents, and discloses an immune lotion for an electrochemical luminescence immunoassay analyzer. The immune lotion comprises a buffer solution and a cleaning solution, wherein the buffer solution is prepared from monopotassium phosphate, tripropylamine, N,N-dibutyl ethanolamine, potassium chloride, polidocanol, polyoxyethylene alkanolamide and hydroxypropyl methyl cellulose, and the pH value of the buffer solution is 6.5 to 7.3; and the cleaning solution is prepared from potassium hydroxide, alkylphenol ethoxylates,lauryl glucoside and Pluronic L81. According to the buffer solution provided by the invention, the concentration of tripropylamine is reduced, and the consistency, stability and accuracy of a detection result are ensured; and the cleaning solution has a more excellent cleaning effect.

Description

technical field [0001] The invention relates to the technical field of in vitro diagnostic reagents for medical devices, in particular to a chemiluminescent buffer solution and a cleaning solution for a Roche automatic immunoassay analyzer. Background technique [0002] Chemiluminescent immunoassay (CLIA) is a new type of labeled immunoassay technology that combines chemiluminescent or bioluminescent systems with immune reactions to detect trace antigens or antibodies. It has the characteristics of rapidity, high sensitivity and good stability, and is easy to standardize, and has been widely used in biology, medical research and clinical laboratory diagnosis. [0003] Cobas e411 / e601 Electrochemiluminescence Immunoassay System of Swiss Roche Company is a fully automatic desktop analyzer using advanced electrochemiluminescence technology. It has been applied to a wide range of measurement items such as thyroid hormone, myocardial injury and tumor markers, infectious diseases...

Claims

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Application Information

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IPC IPC(8): G01N33/53G01N21/76G01N35/00
CPCG01N21/76G01N33/53G01N35/00
Inventor 王旭朱积宝苗远晴
Owner 长春晨裕生物医疗科技有限公司
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