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Primer probe system and kit capable of simultaneously detecting multiple drug-resistant genes

A technology of primer probes and drug-resistant genes, applied in the field of biomedicine, can solve the problems of time-consuming and cumbersome operation processes, and achieve the effects of reducing drug resistance, high application value, and reducing the amount of antibiotics used

Pending Publication Date: 2020-08-28
PILOT GENE TECH HANGZHOU CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, the detection of clinical drug-resistant bacteria is still mainly through the selective culture of specimens to count the number of colonies, and then through the traditional drug sensitivity test. However, due to various reasons such as time-consuming, cumbersome operation, and expensive equipment, it has not been widely used clinically. Therefore, it is extremely important to develop a simple and rapid combined detection method for multiple drug-resistant genes. Great application prospect

Method used

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  • Primer probe system and kit capable of simultaneously detecting multiple drug-resistant genes
  • Primer probe system and kit capable of simultaneously detecting multiple drug-resistant genes
  • Primer probe system and kit capable of simultaneously detecting multiple drug-resistant genes

Examples

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Embodiment 1

[0040] This example discloses a method for simultaneously detecting multiple drug resistance genes. The experimental procedure is as follows:

[0041] Hospital sampling and storage→plasma separation→plasma free nucleic acid extraction→configuration of digital PCR reaction solution→droplet chip generation→amplification→reading→result analysis and report output.

[0042] The instruments and equipment involved are shown in Table 1, and the names and storage conditions of the reagents involved are shown in Table 2.

[0043] Table 1 Instrument and Equipment List

[0044]

[0045]

[0046] Table 2 Reagent names and storage conditions

[0047] Reagent name storage conditions Lysate A Store at 2-8°C Proteinase K Store at 2-8°C magnetic bead suspension Store at 2-8°C Washing solution A Store at 2-8°C Isopropanol Store at room temperature 75% ethanol Store at room temperature eluent Store at 2-8°C

[0048] 1. The exp...

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Abstract

The invention belongs to the field of biological medicines, and particularly relates to a primer probe system and kit capable of simultaneously detecting multiple drug-resistant genes. Firstly, the invention discloses the primer system capable of simultaneously detecting multiple drug-resistant genes, and the primer system comprises a nucleotide sequence group with nucleotide sequences shown in SEQ ID NO: 1-24. The invention also discloses the kit comprising the primer system, and a method for detecting drug-resistant genes by using the primer system or the kit. The kit disclosed by the invention supports high-throughput detection, and can rapidly and accurately detect five bacterial drug-resistant genes with ultrahigh sensitivity, rapidly monitor the change of specific drug-resistant genes in a blood flow infection patient in real time according to quantitative / semi-quantitative detection results of the target drug-resistant genes, timely evaluate the condition of the patient, and provide adjuvant therapy suggestions for clinicians.

Description

technical field [0001] The invention belongs to the field of biomedicine, in particular to a primer-probe system and a kit for simultaneously detecting multiple drug-resistant genes. Background technique [0002] Antibacterial drugs are the most widely used drugs in clinical practice. However, the fatality rate of infectious diseases has increased rapidly in recent years. The reason is the overuse of antibiotics and the difficulty in drug use caused by drug-resistant bacteria. my country is one of the countries where the abuse of antibiotics is the most serious. However, with the increase in the use of antibiotics, bacterial resistance is also becoming more and more serious. At present, the monitoring / detection of bacterial resistance focuses on: 1. Methicillin-resistant Staphylococcus (MRS); 2. Vancomycin-resistant multidrug-resistant Enterococcus (VRE); 3. β-lactam resistance pneumococcus pneumoniae (PRSP) of multidrug-resistant macrolides and macrolides; 4. Gram-negative...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/689C12Q1/14C12Q1/686C12N15/11C12R1/44
CPCC12Q1/689C12Q1/686C12Q2600/16C12Q2563/159C12Q2537/143
Inventor 朱留伟董德坤夏江
Owner PILOT GENE TECH HANGZHOU CO LTD
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