Method for determining repair degree of purple potato extract anthocyanin on oxidative damage in zebra fish bodies

A purple potato, damage repair technology, applied in biochemical equipment and methods, compound screening, detection of programmed cell death, etc., can solve the problem of not evaluating the degree of oxidative damage

Pending Publication Date: 2020-09-01
呼和浩特职业学院
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  • Method for determining repair degree of purple potato extract anthocyanin on oxidative damage in zebra fish bodies
  • Method for determining repair degree of purple potato extract anthocyanin on oxidative damage in zebra fish bodies
  • Method for determining repair degree of purple potato extract anthocyanin on oxidative damage in zebra fish bodies

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Embodiment Construction

[0038] In order to enable those skilled in the art to better understand the technical solution of the present invention, the technical solution of the present invention will be further described below in conjunction with the accompanying drawings and embodiments.

[0039] The method for determining the degree of repair of oxidative damage in zebrafish by anthocyanins from purple potato extracts comprises the following steps,

[0040] S1: Establish zebrafish oxidative damage model;

[0041] Specifically, 7-8hpf (hours after fertilization, hours of post fertilization, hpf) zebrafish embryos and 4dpf (days after fertilization, days of post fertilization, dpf) zebrafish juveniles were randomly divided into blank control group (NC) and lipopolysaccharide treatment group (LPS), placed in a 12-well culture plate, zebrafish 7-8hpf embryos and 4dpf juveniles in the lipopolysaccharide treatment group were oxidatively stressed for 14h and 24h respectively, washed with fresh artificial se...

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Abstract

The invention discloses a method for determining the repair degree of purple potato extract anthocyanin on oxidative damage in zebra fish bodies. The method comprises the following steps: establishinga zebra fish oxidative damage model; then determining the SOD activity and malondialdehyde content of the zebra fish embryos, juvenile fishes and adult fishes after oxidative damage, and judging theoxidative damage strength of the zebra fish; determining main indexes of oxidative damage in the zebra fish body after oxidative damage, the main indexes comprising an ROS generation level, a lipid peroxidation degree and a cell apoptosis degree; and finally, adding a purple potato extract anthocyanin solution into an artificial seawater culture medium for establishing the zebra fish oxidative damage model, carrying out oxidative damage repair on the zebra fish, and determining the strength of the purple potato extract anthocyanin solution on the zebra fish oxidative damage repair function. The determination method provided by the invention can accurately determine the repair degree of the purple potato extract anthocyanin on the oxidative damage in the zebra fish body, and can be appliedto actual repair of the oxidative damage in the zebra fish body.

Description

technical field [0001] The invention relates to the technical field of oxidative damage repair in zebrafish, in particular to a method for measuring the degree of repair of oxidative damage in zebrafish by anthocyanins extracted from purple potatoes. Background technique [0002] Under normal circumstances, there is a dynamic balance of oxidation and anti-oxidation in the animal body, that is, the generation and removal of free radicals in the body maintains a dynamic balance. However, when the body is in a certain special environment, such as changes in food composition, radiation exposure, heavy metal exposure, bacterial and viral infections, emotional and emotional instability, etc., the body can be in a state of oxidative stress. [0003] Anthocyanins are a class of water-soluble pigments widely present in plants. They belong to flavonoid compounds and are powerful antioxidants. They can protect the human body from harmful substances such as free radicals. DNA cleavage,...

Claims

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Application Information

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IPC IPC(8): G01N33/50G01N21/64C12Q1/02C12Q1/26
CPCG01N33/5044G01N21/6486C12Q1/26G01N2500/00
Inventor 韩岚冯雪王玲娜张金然冯永淼
Owner 呼和浩特职业学院
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