Method for preparing glycophosphoglyceride and application of glycophosphoglyceride

A technology of phosphatidyl glucoside and phosphatidyl choline, which is applied in the field of preparation of phosphatidyl glucoside, can solve the problems of not studying the effect of neurodegenerative diseases and difficulty in purifying phosphatidyl glucoside, so as to improve brain Aβ aggregation and reduce behavioral awareness. The effects of knowledge function and broad market application prospect

Active Publication Date: 2020-09-04
OCEAN UNIV OF CHINA +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the importance of phosphatidylglucosides in the brain is known, the effect of ingested phosphatidylglucosides on neurodegenerative diseases has not been studied
At the same time, due to the limitation of separation technology, it is extremely difficult to purify and obtain high-purity phosphatidyl glucoside

Method used

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  • Method for preparing glycophosphoglyceride and application of glycophosphoglyceride
  • Method for preparing glycophosphoglyceride and application of glycophosphoglyceride
  • Method for preparing glycophosphoglyceride and application of glycophosphoglyceride

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] A preparation method of phosphatidyl glucoside, comprising the following steps:

[0027] (1) 100g of phosphatidylcholine is fully mixed with silica gel powder of the same weight;

[0028] (2) 400g of glucose and phospholipase D (200000U) were dissolved in 10L of phosphate buffer;

[0029] (3) Mix the silica gel powder with the above-mentioned phosphate buffer solution and fully stir at 40°C for 12 hours;

[0030] (4) Collect the precipitate by centrifugation, extract 3 times with 1L petroleum ether / ethanol solution (4:1), add 800mL petroleum ether each time and stir for ten minutes;

[0031] (5) Merge the petroleum ether phases and remove the organic solvent by rotary evaporation to obtain high-purity phosphatidylglucosides.

[0032] The phosphatidyl glucoside content of the above product was detected by HPLC-ELSD method, and the phosphatidyl glucoside content was 92.5%.

Embodiment 2

[0034] A preparation method of phosphatidyl glucoside, comprising the following steps:

[0035] (1) 100g of phosphatidylcholine is fully mixed with silica gel powder of the same weight;

[0036] (2) Dissolve 600g trehalose and phospholipase D (200000U) in 10L phosphate buffer;

[0037] (3) Mix the silica gel powder with the above-mentioned phosphate buffer solution and fully stir at 40°C for 12 hours;

[0038] (4) Collect the precipitate by centrifugation, extract 3 times with 1L petroleum ether / ethanol solution (4:1), add 800mL petroleum ether each time and stir for ten minutes;

[0039] (5) Combine the organic phases and remove the organic solvent by rotary evaporation to obtain high-purity phosphatidylglucosides.

[0040] The phosphatidyl glucoside content of the above product was detected by HPLC-ELSD method, and the phosphatidyl glucoside content was 94.6%.

[0041] Wherein, the phosphatidyl glucoside content detection process in embodiment 1 and embodiment 2 is as fol...

Embodiment 3

[0042] Example 3: Evaluation of biological activity of phosphatidyl glucoside and phosphatidyl trehaloside

[0043]Phosphatidyl glucoside and phosphatidyl trehaloside are phospholipid compounds in which glucose and trehalose are linked through a phosphate group at the sn-3 position. The present invention can obtain high-purity phosphatidyl glucoside and phosphatidyl trehaloside through extraction and preparation. After three months of continuous intake of phosphatidyl glucoside and phosphatidyl trehaloside, the APP / PS1 double transgenic Alzheimer's disease can be detected by detection. The cognitive level of disease model mice, Aβ synthesis and Tau protein aggregation, found that it can improve Alzheimer's disease. The specific experimental steps are as follows:

[0044] Experimental objects: 17-week-old male APP / PS1 double transgenic mice, SPF grade, weighing 30-40 grams, 20 mice, raised in a conventional environment.

[0045] Experimental grouping: control group (wild type...

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Abstract

The invention belongs to the technical field of screening of active substances and particularly relates to a method for preparing glycophosphoglyceride and application of the glycophosphoglyceride. According to the method, the glycophosphoglyceride is produced through hydrolyzing phosphatidylcholine with phospholipase D, and meanwhile, carrying out action with a saccharide substrate. Enzyme-methodsynthesis of the glycophosphoglyceride is achieved, and it is confirmed that the obtained glycophosphoglyceride has the capacity for preventing and alleviating Alzheimer's disease and can be used forimproving behavioral and cognitive dysfunction.

Description

Technical field: [0001] The invention belongs to the technical field of active substance screening, and in particular relates to a preparation method and application of phosphatidyl glucoside. Background technique: [0002] Alzheimer's disease (Alzheimer's disease, AD) is a neurodegenerative disease, the most common and most devastating dementia, recognized by the World Health Organization (World Health Organization, WHO) as the primary cause of public health worldwide. One of the problems that affects nearly 50 million people worldwide. The hippocampus and cortex of patients with Alzheimer's disease will shrink, and the most important neuropathological hallmarks are hyperphosphorylation of Tau protein and excessive accumulation of neurofibrillary tangles and amyloid beta (Amyloidbeta, Aβ)-forming amyloid Plaque deposition. In addition, the main pathogenic features are loss of synaptic and neuronal function, reactive gliosis, oxidative and inflammatory damage. Regrettably...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/44C07H15/04C07H1/06A61P25/28
CPCA61P25/28C07H1/06C07H15/04C12P19/44
Inventor 薛长湖刘炎峻徐杰姜晓明李兆杰丛培旭王玉明王静凤
Owner OCEAN UNIV OF CHINA
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