A kind of preparation method and application of epa/ara type plasmalogen

A technology for plasmalogen and enzymolysis of phospholipids, applied in the field of active substance screening, can solve the problems of difficulty in lysophospholipids and plasmalogens, and the inability to completely remove free fatty acids, so as to improve brain Aβ aggregation and reduce behavioral cognitive function , the effect of broad market application prospects

Active Publication Date: 2021-11-12
OCEAN UNIV OF CHINA +1
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Problems solved by technology

[0006] The technical problem to be solved in the present invention is that the current extraction method of plasmalogen includes organic reagent extraction and purification and enzymatic conversion of organic reagent extraction, using the above methods has several problems: the first, free fatty acids cannot be completely removed; second, It is difficult to completely separate lysophospholipids from plasmalogen, and at the same time, it has the disadvantage of solvent residue, which will limit its application range

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  • A kind of preparation method and application of epa/ara type plasmalogen
  • A kind of preparation method and application of epa/ara type plasmalogen
  • A kind of preparation method and application of epa/ara type plasmalogen

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Embodiment 1

[0030] A preparation method of EPA / ARA type plasmalogen, comprising the following steps:

[0031] 1) Freezing and vacuum-drying the mussels to obtain a powder with a fat content of 5-30% through pulverization;

[0032] 2) The powder is subjected to subcritical extraction to obtain a phospholipid complex. The specific operating parameters are the ratio of solvent to solid: 1:1-1.5:1; the working pressure of the extraction tank: 0.3-0.8MPa; the extraction temperature: 30-50°C; the extraction time: 30 -60 minutes; separation tank temperature: 50-70°C,

[0033]3) The mussel phospholipid complex was dissolved in n-hexane, the substrate concentration was controlled at 2 g / mL, the amount of enzyme (phospholipase A1, Lectiase Ultra) added was 4%, the amount of water added was 10%, and the reaction temperature was 45°C in a collector stirrer (200r / min) reaction 20h;

[0034] 4) The reaction solution was centrifuged (4000r / min) and rotary evaporated for 30min to obtain enzymatic phosp...

Embodiment 2

[0041] Embodiment 2: the biological activity evaluation of EPA / ARA type plasmalogen

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Abstract

The invention belongs to the technical field of active substance screening, and relates to a preparation method and application of EPA / ARA type plasmalogens. Continuous hydrolysis of phospholipid complexes with PLA1 enzyme followed by supercritical CO 2 Fluid extraction technology extracts EPA / ARA plasmalogen from enzymatic phospholipids. The EPA / ARA type plasmalogen prepared by the present invention has a purity of more than 80%, and compared with the EPA type plasmalogen or the ARA type plasmalogen, it can significantly reduce the behavioral cognitive function of Alzheimer's disease mice , improving brain Aβ aggregation and Tau protein aggregation, can be used to prepare drugs for preventing and treating diseases such as Alzheimer's disease, and has broad market application prospects.

Description

Technical field: [0001] The invention belongs to the technical field of active substance screening, and in particular relates to a preparation method and application of EPA / ARA type plasmalogen. Background technique: [0002] Alzheimer's disease (Alzheimer's disease, AD) is a neurodegenerative disease, the most common and most devastating dementia, recognized by the World Health Organization (World Health Organization, WHO) as the primary cause of public health worldwide. one of the problems. The hippocampus and cortex of patients with Alzheimer's disease will shrink, and the most important neuropathological hallmarks are hyperphosphorylation of Tau protein and excessive accumulation of neurofibrillary tangles and amyloid beta (Amyloidbeta, Aβ)-forming amyloid Plaque deposition. What's more, Alzheimer's disease has a high mortality rate. Regrettably, current pharmacological treatments for AD are still in their infancy, providing symptomatic relief but not slowing disease ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P9/00A61K31/685A61P25/28
CPCA61K31/685A61P25/28C12P9/00Y02P20/54
Inventor 薛长湖田迎樱刘炎峻徐杰姜晓明李兆杰丛培旭王玉明王静凤
Owner OCEAN UNIV OF CHINA
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