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Application of OAT inhibitor and oncolytic virus in preparation of antitumor drug

An oncolytic virus and inhibitor technology, which is applied in the application field of OAT inhibitors and oncolytic viruses in the preparation of anti-tumor drugs, can solve the problems of uneven sensitivity of M1 virus and unsatisfactory oncolytic effect, and achieve enhanced lytic activity. Tumor effect, high safety effect

Active Publication Date: 2020-09-08
SUN YAT SEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, different tumors have different sensitivities to M1 virus, and for some tumors, when M1 virus is used alone, the oncolytic effect is not ideal.

Method used

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  • Application of OAT inhibitor and oncolytic virus in preparation of antitumor drug
  • Application of OAT inhibitor and oncolytic virus in preparation of antitumor drug

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Example 1 Combined treatment of Dimesna and M1 virus significantly reduces the survival rate of human hepatocellular carcinoma line Hep3B

[0032] Material:

[0033] Human hepatocellular carcinoma Hep3B (purchased from ATCC), M1 virus (preservation number CCTCC V201423), high-glucose DMEM medium (purchased from Corning), multi-functional microplate reader.

[0034] In this embodiment, the OAT inhibitor is Dimesna, whose structural formula is shown in Formula 1:

[0035]

[0036] Formula 1: Dimesna.

[0037] method:

[0038] a) Inoculation of cells, administration treatment: select logarithmic growth phase cells, DMEM complete culture medium (containing 10% fetal bovine serum, 1% double antibody) to make cell suspension, inoculate with the density of 4 × 103 cells in each hole 96-well culture plate. After 12 hours, the cells were completely adhered to the wall. The experiments were divided into control group, Dimesna single use group, M1 infection group and Dimesn...

Embodiment 2

[0044] A pharmaceutical composition for treating tumors, comprising an organic anion transporter subfamily inhibitor and an oncolytic virus.

[0045] It can also be a medicine set for treating tumors, which comprises an OAT inhibitor or a derivative thereof or a combination thereof, and an oncolytic virus. The difference between the medicine set and the composition is that the OAT inhibitor is different from the dosage form of the oncolytic virus, but is packaged independently (for example: in a pill, or capsule, or tablet or ampoule, containing the OAT inhibitor; another pill, or capsules, or tablets, or ampoules, containing oncolytic viruses).

[0046] Oncolytic viruses, OAT inhibitors, and combinations of oncolytic viruses and OAT inhibitors may also contain one or more adjuvants. The adjuvant refers to a component in the composition of the medicine that can assist the curative effect of the medicine. The medicine set can also contain an independently packaged OAT inhibit...

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Abstract

The invention discloses an OAT inhibitor for the first time, and particularly Dimesna can improve the anti-tumor effect of oncolytic virus so as to improve the treatment effectiveness of the oncolyticvirus used as an anti-tumor drug. Cytological experiments prove that the M1 virus and the Dimesna are combined for use, so that morphological lesions of tumor cells can be remarkably caused, and theinhibition effect on the tumor cells is remarkably enhanced.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, and more specifically relates to the application of OAT inhibitors and oncolytic viruses in the preparation of antitumor drugs. Background technique [0002] Oncolytic virus is a kind of replication-competent virus that targets and infects and kills tumor cells without damaging normal cells. Oncolytic virus therapy (oncolytic virotherapy) is an innovative tumor-targeted treatment strategy, which uses natural or genetically engineered viruses to selectively infect tumor cells and replicate in tumor cells to achieve targeted dissolution, The effect of killing tumor cells, but no damage to normal cells. [0003] Oncolytic virus M1 is a Getta-like virus of the Togaviridae alphavirus genus, isolated from mosquitoes of the genus Culex in Hainan Island, China. For example, Chinese invention patent application CN104814984A discloses that oncolytic virus M1 can selectively cause tumor cell death wit...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K45/00A61K31/185A61K35/768A61K35/766A61K35/761A61K35/763A61P35/00
CPCA61K31/185A61K35/761A61K35/763A61K35/766A61K35/768A61K45/00A61P35/00A61K2300/00
Inventor 林园梁剑开蔡静苏兴文
Owner SUN YAT SEN UNIV
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