Production method of flavone-protein self-assembled reversible gel

A production method and self-assembly technology, applied in the field of food processing, can solve the problems of poor water solubility, poor bioavailability, limited application, etc., and achieve the effect of promoting gelation and good stability

Active Publication Date: 2020-09-08
NANCHANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, most flavonoids have poor water solubility and are hydrophobic substances, resulting in poor bioavailability of these compounds, which limits their application in food, medicine and other products

Method used

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Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0014] A method for producing a flavone-protein self-assembled reversible gel, the steps of the preparation method are as follows:

[0015] S1. Weigh 1.0-1.2kg of albumin and dissolve it in 10L of drinking water, and apply an electric field for 10-12 minutes to dissimilate the secondary structure of the protein to obtain isomerized albumin A;

[0016] S2. Weigh 60-65g of naringin and dissolve it in 1L of absolute ethanol to obtain material B;

[0017] S3. Mix isomerized albumin A and material B evenly, incubate in an environment of 85-88°C for 20-24min, take it out and quickly place it in an environment of 1-4°C for 30min, and obtain flavonoid-protein self-assembled reversible coagulation Glue products.

[0018] The intensity of the electric field in step S1 is 20-24V / cm, and the frequency is 20-22Hz.

[0019] The flavone-protein self-assembled reversible gel product described in step S3 can be transformed into a solution under the action of an electric field with a strength...

Embodiment 1

[0021] S1. Weigh 1.0kg of albumin and dissolve it in 10L of drinking water, and apply an electric field for 10 minutes to dissimilate the secondary structure of the protein to obtain isomerized albumin A;

[0022] S2. Weigh 60g of naringin and dissolve it in 1L of absolute ethanol to obtain material B;

[0023] S3. Mix isomerized albumin A and material B evenly, incubate at 85°C for 20 minutes, take it out, and place it at 1°C for 30 minutes to prepare flavonoid-protein self-assembled reversible gel product.

Embodiment 2

[0025] S1. Weigh 1.1 kg of albumin and dissolve it in 10L of drinking water, and apply an electric field for 11 minutes to dissimilate the secondary structure of the protein to obtain isomerized albumin A;

[0026] S2. Weigh 62g of naringin and dissolve it in 1L of absolute ethanol to obtain material B;

[0027] S3. Mix isomerized albumin A and material B evenly, incubate at 86°C for 22 minutes, take it out, and place it at 2°C for 30 minutes to prepare flavonoid-protein self-assembled reversible gel product.

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Abstract

The invention discloses a production method of flavone-protein self-assembly reversible gel. The flavone-protein self-assembly reversible gel is obtained by adding naringin into isomerized albumin byutilizing the characteristic that the secondary structure of the albumin can be isomerized under the action of an electric field and a thermal driving effect so as to strengthen the action of hydrogenbonds and p-pi bonds and promote the gelation of the albumin. The gel prepared by the method provided by the invention has good stability and reversibility, that is to say, the gel is changed into asolution under the action of an electric field, and the solution can be changed into gel again through incubation.

Description

technical field [0001] The invention relates to the field of food processing, in particular to a production method of flavonoid-protein self-assembled reversible gel. Background technique [0002] Albumin is the most abundant protein in plasma protein, accounting for about 60% of plasma protein content. At room temperature, in the albumin molecule, the α-helix is ​​the main structure, accounting for about 67%. Flavonoids are compounds composed of two benzene rings with phenolic hydroxyl groups connected to each other through the central three-carbon atom, which can effectively remove oxygen free radicals in the body and prevent cell degeneration and aging. Flavonoids also have the effects of improving blood circulation, lowering cholesterol, and improving symptoms of cardiovascular diseases. However, most flavonoids have poor water solubility and are hydrophobic substances, resulting in poor bioavailability of these compounds, which limits their application in food, medici...

Claims

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Application Information

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IPC IPC(8): C08J3/075C08L89/00C08K5/1545
CPCC08J3/075C08J2389/00C08K5/1545
Inventor钟业俊杨雷
OwnerNANCHANG UNIV