Reagent for digesting nucleic acid pollution, and preparation method and application of reagent

A technology for digesting nucleic acid and reagents, which is applied in the field of reagents for digesting nucleic acid contamination and its preparation, can solve problems such as toxicity and use restriction factors, and achieve the effects of non-toxic use, wide application range, and short processing time

Active Publication Date: 2020-09-15
湖北擎科生物科技有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are many dead ends and barriers in ultraviolet radiation, such as the inability to do anything about reagent solutions placed in cabinets or bottles, and ozone generated by ultraviolet radiation is toxic to the human body
[0005] Nuclease treatment of nucleic acid contamination is also a relatively common way, but usually limited to the treatment of nucleic acid contamination in the buffer
Therefore, there are limiting factors or disadvantages in the existing above-mentioned different methods

Method used

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  • Reagent for digesting nucleic acid pollution, and preparation method and application of reagent
  • Reagent for digesting nucleic acid pollution, and preparation method and application of reagent
  • Reagent for digesting nucleic acid pollution, and preparation method and application of reagent

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Embodiment 1

[0038] The reagent for digesting nucleic acid contamination in this embodiment consists of liquid A and liquid B, wherein liquid A is a hydrogen peroxide solution with a mass content of 0.3%, and liquid B is CuSO 4 and Tris mixed solution, CuSO in the mixed solution 4 The concentration of Tris was 1 mM, the concentration of Tris was 4 mM, and the pH value of the mixed solution was 7.5.

[0039] The preparation method of the above-mentioned reagent for digesting nucleic acid contamination is as follows:

[0040] 1. Preparation of liquid A

[0041] Use ultrapure water to dilute the 30% hydrogen peroxide mother solution to a mass concentration of 0.3%, that is, add 99 parts of ultrapure water to 1 part of the 30% hydrogen peroxide mother solution, mix well, and obtain liquid A.

[0042] 2. Preparation of liquid B

[0043] Weigh 0.25g of copper sulfate pentahydrate and 0.49g of Tris, add to 1L of ultrapure water, mix well, and adjust the pH to 7.5 to prepare B solution.

Embodiment 2

[0045] The reagent for digesting nucleic acid contamination in this embodiment consists of liquid A and liquid B, wherein liquid A is a hydrogen peroxide solution with a mass content of 0.2%, and liquid B is CuSO 4 and Tris mixed solution, CuSO in the mixed solution 4 The concentration of Tris is 0.5mM, the concentration of Tris is 5mM, and the pH value of the mixed solution is 7.0.

[0046] The preparation method of the above-mentioned reagent for digesting nucleic acid contamination is as follows:

[0047] 1. Preparation of liquid A

[0048] Dilute the 30% hydrogen peroxide mother solution to a mass concentration of 0.2% with ultrapure water to obtain liquid A.

[0049] 2. Preparation of liquid B

[0050] Add CuSO to 1L of ultrapure water 4 and Tris, making CuSO 4 The concentration of Tris was 0.5mM, the concentration of Tris was 5mM, mixed evenly, and the pH was adjusted to 7.0 to prepare B solution.

Embodiment 3

[0052] The reagent for digesting nucleic acid contamination in this embodiment consists of liquid A and liquid B, wherein liquid A is a hydrogen peroxide solution with a mass content of 0.4%, and liquid B is CuSO 4 and Tris mixed solution, CuSO in the mixed solution 4 The concentration of Tris was 1.5 mM, the concentration of Tris was 3 mM, and the pH value of the mixed solution was 8.0.

[0053] The preparation method of the above-mentioned reagent for digesting nucleic acid contamination is as follows:

[0054] 1. Preparation of liquid A

[0055] Dilute the 30% hydrogen peroxide mother solution to a mass concentration of 0.4% with ultrapure water to obtain liquid A.

[0056] 2. Preparation of liquid B

[0057] Add CuSO to 1L of ultrapure water 4 and Tris, making CuSO 4 The concentration of Tris was 1.5 mM, the concentration of Tris was 3 mM, mixed well, and the pH was adjusted to 8.0 to obtain liquid B.

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Abstract

The invention provides a reagent for digesting nucleic acid pollution, and a preparation method and application of the reagent. The reagent for digesting nucleic acid pollution comprises a solution Aand a solution B, wherein the solution A is a hydrogen peroxide solution with a mass content of 0.2-0.4%, the solution B is a mixed solution of metal ions and a buffer agent, the concentration of themetal ions in the mixed solution is 0.5-1.5 mM, the concentration of the buffer agent is 3-5 mM, and the pH value of the mixed solution is 7.0-8.0. The reagent provided by the invention can flexibly treat various nucleic acid pollutants in reagents, buffer solutions and equipment in laboratories, and has the advantages of short treatment time, good nucleic acid pollution elimination effect, no toxicity in use and wide application range.

Description

technical field [0001] The invention relates to a reagent, in particular to a reagent for digesting nucleic acid contamination and its preparation method and application. Background technique [0002] In recent years, nucleic acid detection technology has been gradually promoted and applied in my country. It has the characteristics of high sensitivity, and at the same time, the laboratory is also prone to pollution. At present, common ways for laboratories to eliminate nucleic acid contamination include high-pressure steam sterilization, ultraviolet irradiation, and nuclease treatment. [0003] The use of high temperature and high pressure steam sterilization can inactivate the microorganisms in the buffer of the kit and degrade the nucleic acid to remove contamination; however, this method can only treat reagents and buffers, and cannot handle the residual nucleic acid contamination in the laboratory environment and experimental equipment. And some reagents cannot be proce...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68C12Q1/6844
CPCC12Q1/68
Inventor 卢晋华曹文刚
Owner 湖北擎科生物科技有限公司
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