Construction method and application of SDK2 gene mutation mice mice

Abstract

The invention provides a construction method of an SDK2 gene mutation mice model. The mice model comprises the following steps of designing and constructing sgRNA capable of specifically identifying an SDK2 gene on the basis of a CRISPR / Cas9 system, injecting the sgRNA and a targeting vector constructed on the basis of the sgRNA into a mice fertilized egg; and after embryo transplantation, screening out F0-generation mice with SDK2 gene mutation from the output mice, and hybridizing the F0-generation mice with wild type mice to obtain an F1-generation mice model with SDK2 gene mutation. The mice model constructed by the method can be stably passaged, the action mechanism of the SDK2 gene in mice hereditary cataract can be conveniently researched in practical application, and under the condition that human patient research materials are not easy to obtain and are restricted by medical ethics, the SDK2 gene can be stably cloned. The mice model provided by the invention can become an important tool in the research of hereditary cataract, and a research model capable of realizing stable heredity is provided in the research of pathogenesis, treatment methods, drug screening, cataract surgery and the like.

Description

Technical field [0001] The invention belongs to the technical field of animal model construction, and in particular relates to a method for constructing an SDK2 gene mutation mouse model and its application. Background technique [0002] Congenital cataract is the first blinding eye disease in children. Globally, 200,000 children are blinded by cataract, which accounts for about 5-20% of children’s blindness. Congenital cataracts have many and complex causes, which can be divided into three categories: genetic factors, environmental factors and unknown causes. Studies have confirmed that genetic factors are the most important pathogenic factor, accounting for about 50% of the disease. More than 40 pathogenic genes have been found to be related to autosomal dominant cataract (Cat-Map; http: / / cat-map.wustl.edu / ), including 13 lens protein genes and 7 membrane protein genes ( Gap link protein and channel protein, etc.), 6 developmental and transcription factor genes, 3 cytoskeletal...

AI Technical Summary

Problems solved by technology

Since there have been no reports of congenital cataracts caused by the cell adhesion molecule SDK2 at home and abroad, the pathogenic mechanism is still unclear, and the corresponding treatment methods have not been systematically studied.

However, the ocular materials of human patients are difficult to obtain and the reproducibility is poor, which restricts the development of related research.

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