Sheath fluid for urine analyzer and preparation method thereof

A technology of urinalysis and sheath fluid, which is applied in the field of sheath fluid, can solve the problems of heating and dissolving, containing organic solvents, etc., and achieves the effects of low corrosion, simple operation, and small environmental impact

Pending Publication Date: 2020-10-16
DIRUI MEDICAL TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to solve the technical problem in the prior art that the sheath fluid for urine analyzer c

Method used

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  • Sheath fluid for urine analyzer and preparation method thereof
  • Sheath fluid for urine analyzer and preparation method thereof
  • Sheath fluid for urine analyzer and preparation method thereof

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preparation example Construction

[0033] The present invention also provides a method for preparing the above-mentioned sheath fluid for urine analyzers: according to the ratio, osmotic pressure compensator, chelating agent, surfactant, particle stabilizer, antibacterial agent and buffer are dissolved in water, and the pH range is adjusted to 6- 8. Filter with a microporous membrane to obtain the sheath fluid for the urine analyzer.

[0034] In the above technical solution, the pH is adjusted by HCl or NaOH; the pore size of the microporous membrane is 0.05-0.5 μm, preferably 0.1 μm. The order of dissolving the osmotic pressure compensator, chelating agent, surfactant, particle stabilizer, antibacterial agent and buffer in water is not particularly limited. Usually, the buffer solution is firstly prepared into buffer solution, or directly used containing buffer water, and then dissolve the osmotic pressure compensator, chelating agent, surfactant, particle stabilizer and antibacterial agent in it.

[0035] Th...

Embodiment 1

[0040] Sheath fluid for urine analyzer, the formula is as follows:

[0041]

[0042]

[0043] The preparation method of the above-mentioned sheath fluid for urine analyzer: mix 800mL of 35mmol / L acetate buffer solution with a pH of 6.8 and 200mL of 35mmol / L phosphate buffer solution, add sodium chloride, potassium chloride, ethylenediaminetetra Disodium acetate, poloxamer, sodium hyaluronate and sodium pyrithione are completely dissolved, adjusted to pH 6.8 with HCl and NaOH, and filtered with a 0.1 μm microporous membrane to obtain the required urine analyzer. The sheath fluid has an osmotic pressure of 280Osm / kg.

[0044] The urine analyzer of embodiment 1 is combined with the sheath fluid and the instrument to detect the cells, and the results are as follows: Figure 1-3 As shown, it can be seen that each cell is classified correctly and the picture is clear.

Embodiment 2

[0046] Sheath fluid for urine analyzer, the formula is as follows:

[0047] components concentration content Phosphate buffer 25mmol / L 1L potassium chloride 3g / L 3g Sodium chloride 3g / L 3g Disodium edetate 0.08g / L 0.08g Tween 20 0.5％ 5ml Sphingomyelin 0.02g / L 0.02g ProClin300 0.1％ 1ml

[0048] The preparation method of the above-mentioned sheath fluid for the urine analyzer: pH 7.0 25mmol / L phosphate buffer 1L, add sodium chloride, potassium chloride, edetate disodium, Tween 20, sphingomyelin and ProClin300 respectively Until the dissolution is complete, adjust the pH to 7.0 with HCl and NaOH, and filter with a 0.1 μm microporous membrane to obtain the required sheath fluid for a urine analyzer with an osmotic pressure of 285 Osm / kg.

[0049] The urine analyzer of embodiment 2 is jointly detected with the sheath fluid and the instrument, and the results are as follows Figure 4-5 , it can be seen that eac...

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Abstract

The invention discloses sheath fluid for a urine analyzer and a preparation method thereof, which belong to the technical field of sheath fluid. The technical problem that sheath fluid for a urine analyzer in the prior art contains an organic solvent and needs to be heated and dissolved is solved. The sheath fluid for the urine analyzer comprises water, an osmotic pressure compensating agent, a chelating agent, a surfactant, a particle stabilizer and an antibacterial agent, the pH range is 6-8, and the osmotic pressure range is 220-340 mOsm/kg; the particle stabilizer is one or more of sodiumhyaluronate, sphingomyelin, adenine, aprotinin, trehalose, glucose and the like, and in the sheath fluid, the mass concentration of the particle stabilizer is less than or equal to 4g/L. The sheath fluid for the urine analyzer can form sheath flow without changing the particle form, the corrosion to a detection device is small, and a sample wrapped by the sheath fluid not only enables the detection device to count particles, but also can be shot and imaged at a high speed.

Description

technical field [0001] The invention belongs to the technical field of sheath fluid, and in particular relates to a sheath fluid for a urine analyzer and a preparation method thereof. Background technique [0002] The flowing liquid can be divided into two states of laminar flow and turbulent flow, and the flowing liquid has a boundary point, which is the Reynolds number Re. Re=dρv / η, where d is the inner diameter of the tube, ρ is the liquid density, v the liquid flow rate, and η is the viscosity coefficient. When Re<2300, the liquid flow is in the state of laminar flow, and when Re>2300, the liquid flow is in the state of turbulent flow. The test instrument requires the sample to be in a laminar flow state, and the flow rate is usually controlled within 10m / s. During the test, the sample is covered with a layer of sheath fluid with a higher flow rate, so that the particles in the sample do not diverge, and a stable coaxial flow state between the sample and the shea...

Claims

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Application Information

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IPC IPC(8): G01N15/14G01N33/493G01N21/00G01N35/00
CPCG01N15/14G01N33/493G01N21/00G01N35/00Y02A50/30
Inventor 林月王嘉星
Owner DIRUI MEDICAL TECH CO LTD
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