Morchella mycelium culture medium and preparation method thereof

A technology of mycelium and morel, applied in botany equipment and methods, cultivation, plant cultivation, etc., can solve the problem of wild morel resources being on the verge of depletion, achieve growth efficiency and quality, simple preparation method, get simple effects

Inactive Publication Date: 2020-11-17
南京康之春生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Due to the increasing use of morels and the predatory acquisition of wild morels in my country by Japan and South Korea, the resources of wild morels are on the verge of depletion; while the research on morels in my country has just started, At this stage, the suitable mother culture medium, liquid medium formula and artificial cultivation technology for morel bacteria still need to be improved urgently

Method used

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  • Morchella mycelium culture medium and preparation method thereof

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Comparison scheme
Effect test

Embodiment 1

[0020] See figure 1 , The present embodiment provides a technical solution: A Mycelium medium was prepared from the following raw materials by weight: 180g of potato, glucose 15g, agar 15g, 1g yeast extract, 1g of magnesium sulfate and water 900g.

[0021] Then medium was prepared as follows:

[0022] Sl, after taking peeled potato 180g, 900g into the water, weighed to obtain the weight value of 1080 g, was boiled for 30 ~ 60min, filtered juice, water added to return the weight to 1080 g, obtaining an extract of potato 1080g.

[0023] S2, was added into the extract of potato dextrose, agar, yeast extract, magnesium sulfate, Simmer solution, while stirring until dissolved, length of 10 ~ 15min while stirring to obtain a culture medium.

[0024] S3, the culture medium dispensed in a test tube and sealed.

[0025] S4, autoclaving of the tube, and the pressure is controlled at 0.6 ~ 0.7kg, sterilization time is 60 ~ 120min, after sterilization is completed, is stored in a sterile envi...

Embodiment 2

[0030] The present embodiment provides a technical solution: A Mycelium medium was prepared from the following raw materials by weight: 190g of potato, glucose 15g, agar 15g, 1g yeast extract, 1g of magnesium sulfate and water 950g.

[0031] Then medium was prepared as follows:

[0032] Sl, after taking peeled potato 190g, 950g into the water, weighed to obtain the weight value of 1140 g, was boiled for 30 ~ 60min, filtered juice, water added to return the weight to 1140 g, obtaining an extract of potato 1140g.

[0033] S2, was added into the extract of potato dextrose, agar, yeast extract, magnesium sulfate, Simmer solution, while stirring until dissolved, length of 10 ~ 15min while stirring to obtain a culture medium.

[0034] S3, the culture medium dispensed in a test tube and sealed.

[0035] S4, autoclaving of the tube, and the pressure is controlled at 0.6 ~ 0.7kg, sterilization time is 60 ~ 120min, after sterilization is completed, is stored in a sterile environment, the tu...

Embodiment 3

[0040] The present embodiment provides a technical solution: A Mycelium medium was prepared from the following raw materials by weight: potato 200g, dextrose 20g, agar 20g, 1.2g yeast extract, 1.2g of magnesium sulfate and 1000g water .

[0041] Then medium was prepared as follows:

[0042] Sl, peeled potatoes 200g after taking, into 1000g of water, 1200 g of weighed to obtain a weight value, boiled for 30 ~ 60min, filtered juice, water was added to 1200 g of the restored weight, of potato extract to obtain 1200g.

[0043] S2, was added into the extract of potato dextrose, agar, yeast extract, magnesium sulfate, Simmer solution, while stirring until dissolved, length of 10 ~ 15min while stirring to obtain a culture medium.

[0044] S3, the culture medium dispensed in a test tube and sealed.

[0045] S4, autoclaving of the tube, and the pressure is controlled at 0.6 ~ 0.7kg, sterilization time is 60 ~ 120min, after sterilization is completed, is stored in a sterile environment, the...

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Abstract

The invention is applicable to the technical field of morchella culture, and provides a morchella mycelium culture medium and a preparation method thereof. The morchella mycelium culture medium is prepared from potatoes, glucose, agar, yeast extract, magnesium sulfate and water, peeled potatoes are taken and put into water, a weight value A is obtained through weighing, boiling is conducted for 30-60 min, filtering is conducted, juice is taken, water is supplemented, and the weight is restored to the value A; and potato extract is obtained, glucose, agar, yeast extract and magnesium sulfate are added into the potato extract, are boiled with slow fire to be molten, and meanwhile are stirred until dissolving is achieved to obtain the culture medium, the culture medium is subpackaged into test tubes, sealing is carried out, the test tubes are sterilized finally, and the sterilized test tubes are stored in a sterile environment to obtain the culture medium to be used. By means of the culture medium and the preparation method, materials are easy to obtain, the preparation method of the culture medium is simple, sufficient nutrient substances can be provided for culture of morchella esculenta, and the growth efficiency and quality of morchella esculenta hyphae are effectively improved.

Description

Technical field [0001] The present invention belongs to the technical field morel cultivation, Mycelium particularly to a method for its preparation medium. Background technique [0002] Morel is a valuable perennial large medicinal fungi, because most of the growth and fruiting body tan named on mulberry plants. Morel was first seen in drug efficacy, "Compendium of Materia Medica", "resistance" records Morel bitter, cold. In traditional Chinese medicines for the treatment of diarrhea, night sweats, vaginal bleeding, blood prolapse diarrhea and other symptoms. New research shows that Morel also has a unique anti-cancer effect, is internationally recognized biological agents in cancer has the highest efficiency of a medicinal fungus. The main pharmacologically active ingredient Morel Morchella polysaccharide is, for the main purpose of the Morel Morchella cultured obtaining a polysaccharide formulation for the production of medicine or health care products, typically by Morchella ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G18/20C05G3/00
CPCA01G18/20C05D5/00C05G3/00C05F11/00C05F11/08
Inventor 许忠许腾龙金媛媛李娟朱荣荣邵丽赵明文蒋宁王继红
Owner 南京康之春生物科技有限公司
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