Novel preservation solution for microbial nucleic acid in cerebrospinal fluid

A technology for microorganisms and cerebrospinal fluid, applied in the field of molecular biology, can solve the problems of poor stability of microbial nucleic acids, inability to cryopreservation, and immediate inspection, and achieve the effects of avoiding cell lysis, preventing cell lysis and improving accuracy.

Pending Publication Date: 2020-11-17
广州源古纪科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, cerebrospinal fluid specimens need to be sent for inspection immediately after collection (no more than 1 hour), and they cannot be stored at low temperature. The stability of microbial nucleic acids in cerebrospinal fluid is poor

Method used

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  • Novel preservation solution for microbial nucleic acid in cerebrospinal fluid
  • Novel preservation solution for microbial nucleic acid in cerebrospinal fluid

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Embodiment 1

[0019] A new preservation solution for microbial nucleic acid in cerebrospinal fluid, including 10mmol / L nuclease inhibitor ethylenediaminetetraacetic acid, 20mmol / L buffer solution guanidine hydrochloride buffer solution, 15mmol / L protein denaturation inhibitor sodium dodecylsulfonate, 10mmol / L trisodium citrate, 25mmol / L bacteriostatic agent potassium sorbate, 5mmol / L stabilizer sodium acetate; 10% isopropanol.

Embodiment 2

[0021] A new preservation solution for microbial nucleic acid in cerebrospinal fluid, including 5mmol / L nuclease inhibitor ethylenediaminetetraacetic acid, 30mmol / L buffer solution guanidine hydrochloride buffer solution, 20mmol / L protein denaturation inhibitor sodium dodecylsulfonate, 5mmol / L trisodium citrate, 20mmol / L bacteriostatic agent potassium sorbate, 10mmol / L stabilizer sodium acetate; 5% isopropanol.

Embodiment 3

[0023] A new preservation solution for microbial nucleic acid in cerebrospinal fluid, including 18mmol / L nuclease inhibitor ethylene glycol tetraacetic acid, 15mmol / L buffer solution guanidine hydrochloride buffer solution, 25mmol / L protein denaturation inhibitor sodium dodecylsulfonate, 8mmol / L trisodium citrate, 30mmol / L antibacterial agent potassium sorbate, 6mmol / L stabilizer glycine; 8% isopropanol.

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Abstract

The invention discloses a novel preservation solution for microbial nucleic acid in cerebrospinal fluid. The fluid comprises the following components of a nuclease inhibitor, a buffer solution, a protein denaturation inhibitor, trisodium citrate, a bacteriostatic agent and a stabilizer, and the pH value of the novel preservation solution for the microbial nucleic acid in the cerebrospinal fluid is7-8. According to the novel preservation solution for the microbial nucleic acid in the cerebrospinal fluid, the activity of nuclease can be rapidly and effectively inhibited, the nucleic acid in a cerebrospinal fluid sample is prevented from being degraded, the content of the nucleic acid in the cerebrospinal fluid is guaranteed, and meanwhile, the cell lysis is avoided, the detection backgroundnoise is reduced, and the accuracy of cerebrospinal fluid detection is improved; and the preservation fluid is relatively simple in components and low in cost, and meanwhile, the stability of the free nucleic acid can be maintained at room temperature, and the fluid is extremely suitable for wide clinical application.

Description

technical field [0001] The invention relates to the technical field of molecular biology, in particular to a novel preservation solution of microbial nucleic acid in cerebrospinal fluid. Background technique [0002] Cerebrospinal fluid is a colorless and transparent liquid that exists in the ventricle and subarachnoid space. Cerebrospinal fluid surrounds and supports the entire brain and spinal cord, effectively reducing the weight of the brain to 1 / 6, and protecting it from trauma. In clearing metabolites and inflammatory exudates, it plays the role of lymph fluid in other parts of the body. Cerebrospinal fluid is continuously produced and absorbed back into the veins. It plays the role of lymph in the central nervous system. It supplies certain nutrients to brain cells, removes metabolites from brain tissue, regulates the acid-base balance of the central nervous system, and buffers Pressure on the brain and spinal cord, has a protective and supportive effect on the brain...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6806
CPCC12Q1/6806C12Q2527/127C12Q2527/125
Inventor 段志峰
Owner 广州源古纪科技有限公司
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