Amniotic fluid protein powder, preparation method thereof and application of amniotic fluid protein powder
A protein powder, amniotic fluid technology, applied in the application, food freezing, multi-step food processing and other directions, can solve the inactivation of amniotic fluid components thermal decomposition active substances, amniotic fluid restricted edible and market acceptability, unfavorable food processing, preservation and other problems. Food and other problems to achieve the effect of enhancing immunity, solving the problem of deodorization, and expanding applications
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Embodiment 1
[0025] The present embodiment provides a method for preparing avian amniotic fluid protein powder. The birds include chickens, ducks, geese, quails and other bird species, and the specific steps include:
[0026] (1) Fractional purification of avian amniotic fluid
[0027] After the avian amniotic fluid was extracted with a syringe, centrifuge at 10,000 rpm / min for 15 min at 4 °C, and the supernatant was taken for use;
[0028] The supernatant is further separated and purified through a filter membrane with a pore size of 200KDa and a molecular weight, and the filtrate is used for later use. The separation and purification in this step can enrich and concentrate the macromolecular active protein in the amniotic fluid.
[0029] The filtrate is further separated and purified through a filter membrane with a pore size of 5KDa and a molecular weight, and the retentate is taken for later use. The separation and purification of this step can remove water, small molecular salts, mine...
Embodiment 2
[0037] The present embodiment provides a preparation method of avian amniotic fluid protein powder, and the specific steps include:
[0038] (1) Fractional purification of avian amniotic fluid
[0039] After extracting the amniotic fluid of birds with a syringe, centrifuge at 11000rpm / min at 4°C for 10min, and take the supernatant for use;
[0040] The supernatant is further separated and purified through a filter membrane with a pore size of 200KDa and a molecular weight, and the filtrate is used for later use. The separation and purification in this step can enrich and concentrate the macromolecular active protein in the amniotic fluid.
[0041] The filtrate is further separated and purified through a filter membrane with a pore size of 5KDa and a molecular weight, and the retentate is taken for later use. The separation and purification of this step can remove water, small molecular salts, minerals and lipids in the amniotic fluid.
[0042] (2) Addition of excipients
[0...
Embodiment 3
[0049] The present embodiment provides a preparation method of avian amniotic fluid protein powder, and the specific steps include:
[0050] (1) Fractional purification of avian amniotic fluid
[0051] After avian amniotic fluid was extracted with a syringe, centrifuge at 12,000 rpm / min for 5 min at 4°C, and the supernatant was taken for later use;
[0052]The supernatant is further separated and purified through a filter membrane with a pore size of 200KDa and a molecular weight, and the filtrate is used for later use. The separation and purification in this step can enrich and concentrate the macromolecular active protein in the amniotic fluid.
[0053] The filtrate is further separated and purified through a filter membrane with a pore size of 5KDa and a molecular weight, and the retentate is taken for later use. The separation and purification of this step can remove water, small molecular salts, minerals and lipids in the amniotic fluid.
[0054] (2) Addition of excipien...
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