Metabolic marker of myeloblastoma in urine and application thereof
A technology of medulloblastoma and metabolites, which is applied in the field of metabolic markers of medulloblastoma in urine and its early diagnosis and postoperative disease monitoring
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0039] Example 1: Research on Early Specific Diagnostic Markers of Medulloblastoma
[0040] Below in conjunction with accompanying drawing, further illustrate the present invention through the embodiment, but not as limitation to the present invention. Specific materials and their sources used in embodiments of the invention are provided below. However, it should be understood that these are merely exemplary and not intended to limit the present invention, and materials identical or similar to the type, model, quality, property or function of the following reagents and instruments can be used to implement the present invention. The experimental methods used in the following examples are conventional methods unless otherwise specified. The materials and reagents used in the following examples can be obtained from commercial sources unless otherwise specified.
[0041] We used liquid chromatography-high resolution mass spectrometry (LC-MS) to detect metabolites in urine throug...
Embodiment 2
[0072] Example 2: Research on markers for disease monitoring of medulloblastoma after surgery
[0073] Materials and Reagents
[0074] 1) Instrument: Waters H-class liquid chromatograph (waters company) LTQ-Orbitrap velos pro mass spectrometer (Thermofisher Scientific company).
[0075] 2) Main reagents: acetonitrile (Thermofisher Scientific Company); C18 reverse-phase chromatographic column (3.0 mm×100 mm, C18, 1.7 μm, Waters Company).
[0076] 3) Samples: preoperative urine samples of 112 patients (67 males, 45 females) with medulloblastoma, urine samples of 80 patients (51 males, 29 females) with medulloblastoma one month after operation Fluid samples; urine from 118 age- and gender-matched normal control groups (67 males and 51 females); from Beijing Tiantan Hospital. The average age of each group is not more than 2 years old.
[0077] 2.1 Collection of human urine samples
[0078] Collect fasting morning urine and centrifuge at 5000g for 30min to remove sediment.
[...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com