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A kind of bee pollen lipid extract and its extraction method and application

An extraction method, technology of bee pollen, applied in the field of natural herbal preparations to achieve good preventive/preventive effect

Active Publication Date: 2022-04-19
BEE RES INST CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, bee pollen is mainly used as a functional food for human consumption, and there are no reports on the extraction of lipids and its preventive and therapeutic functions.

Method used

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  • A kind of bee pollen lipid extract and its extraction method and application
  • A kind of bee pollen lipid extract and its extraction method and application
  • A kind of bee pollen lipid extract and its extraction method and application

Examples

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Embodiment 1

[0037] The present embodiment provides a method for preparing camellia bee pollen fat extract, and the specific steps are as follows:

[0038] 1) Freeze-dry the fresh camellia bee pollen in a vacuum freeze dryer, then pulverize it with a traditional Chinese medicine pulverizer, pass through a 50-300 mesh sieve to obtain bee pollen powder, and mix according to the ratio of material to liquid (g / mL) 1:1.5 The ratio is fully dissolved in deionized water by vortex shaking to form an aqueous solution of bee pollen;

[0039] 2) Add an extractant to the bee pollen aqueous solution at a volume ratio of 1:4, and mix thoroughly to obtain a bee pollen mixed solution; the extractant is formed by mixing methanol and dichloromethane at a volume ratio of 1:2;

[0040] 3) Ultrasound-assisted extraction of the bee pollen mixed solution in an ice-water bath at 0-4°C for 40 minutes, with an ultrasonic power of 100KHz, and centrifugation in a refrigerated centrifuge at 4°C, 3000rpm, for 15 minute...

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Abstract

The invention belongs to the technical field of natural herbal preparations, and in particular relates to a bee pollen lipid extract and its extraction method and application. The extraction method of the bee pollen lipid extract uses an extractant to extract the bee pollen aqueous solution; the extractant is composed of methanol and methylene chloride at a volume ratio of 1:(1-3). The present invention finds that the mixed liquid obtained by mixing methanol and dichloromethane in a specific volume ratio is used as an extractant for the extraction of bee pollen lipid extract, which not only can ensure the safety of the extract, but also has the effect on the functional polar lipids in bee pollen. With remarkable extraction selectivity, it can greatly increase the content of functional polar lipids in bee pollen, and at the same time reduce the degree of extraction of weaker polar triglycerides and long-chain saturated fatty acids, so that bee pollen lipids can be extracted The compound has remarkable efficacy in preventing / treating acute pneumonia.

Description

technical field [0001] The invention belongs to the technical field of natural herbal preparations, and in particular relates to a bee pollen lipid extract and its extraction method and application. Background technique [0002] Acute lung injury (Acute Lung Injury, ALI) is a systemic inflammatory response syndrome, clinically manifested as acute respiratory distress in the lungs (Acute Respiratory Distress Syndrome, ARDS) Capillary endothelium and alveolar epithelium injury, and increased vascular permeability cause acute, progressive dyspnea, refractory hypoxemia, and pulmonary edema. Clinically, control of the primary disease and anti-infection treatment, mechanical ventilation support treatment, and drug (such as adrenocortical hormone) treatment are usually used to control the further development of the disease. [0003] Although drug therapy has achieved positive therapeutic effects in many patients with acute pneumonia, there are still a considerable number of patien...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K35/64A61K36/82A61P11/00
CPCA61K35/64A61K36/82A61P11/00A61K2236/331A61K2236/55A61K2236/51
Inventor 李强强吴黎明王凯薛晓锋
Owner BEE RES INST CHINESE ACAD OF AGRI SCI
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