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Bee pollen lipid extract, and extraction method and application thereof

An extraction method and bee pollen technology are applied in the field of natural botanical preparations to achieve good preventive/preventive effects

Active Publication Date: 2020-12-04
BEE RES INST CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, bee pollen is mainly used as a functional food for human consumption, and there are no reports on the extraction of lipids and its preventive and therapeutic functions.

Method used

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  • Bee pollen lipid extract, and extraction method and application thereof
  • Bee pollen lipid extract, and extraction method and application thereof
  • Bee pollen lipid extract, and extraction method and application thereof

Examples

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Embodiment 1

[0037] The present embodiment provides a method for preparing camellia bee pollen fat extract, and the specific steps are as follows:

[0038] 1) Freeze-dry the fresh camellia bee pollen in a vacuum freeze dryer, then pulverize it with a traditional Chinese medicine pulverizer, pass through a 50-300 mesh sieve to obtain bee pollen powder, and mix according to the ratio of material to liquid (g / mL) 1:1.5 The ratio is fully dissolved in deionized water by vortex shaking to form an aqueous solution of bee pollen;

[0039] 2) Add an extractant to the bee pollen aqueous solution at a volume ratio of 1:4, and mix thoroughly to obtain a bee pollen mixed solution; the extractant is formed by mixing methanol and dichloromethane at a volume ratio of 1:2;

[0040] 3) Ultrasound-assisted extraction of the bee pollen mixed solution in an ice-water bath at 0-4°C for 40 minutes, with an ultrasonic power of 100KHz, and centrifugation in a refrigerated centrifuge at 4°C, 3000rpm, for 15 minute...

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Abstract

The invention belongs to the technical field of natural plant medicine preparations, and particularly relates to a bee pollen lipid extract, and an extraction method and application thereof. The extraction method of the bee pollen lipid extract is as follows: bee pollen aqueous solution is extracted by utilization of an extracting agent; and the extracting agent is composed of methanol and dichloromethane according to a volume ratio of 1:(1-3). According to the invention, mixed solution obtained by mixing methanol and dichloromethane according to the specific volume ratio is used as the extracting agent for extracting the bee pollen lipid extract; therefore, the safety of the extract can be ensured; furthermore, the extracting selectivity on functional polar lipid in bee pollen is remarkable; the content of the functional polar lipid in the bee pollen can be increased to a greater extent; and meanwhile, the extraction degree of triglyceride and long-chain saturated fatty acid with weaker polarity is reduced, so that the bee pollen lipid extract has a remarkable effect of preventing / treating acute pneumonia.

Description

technical field [0001] The invention belongs to the technical field of natural herbal preparations, and in particular relates to a bee pollen lipid extract and its extraction method and application. Background technique [0002] Acute lung injury (Acute Lung Injury, ALI) is a systemic inflammatory response syndrome, clinically manifested as acute respiratory distress in the lungs (Acute Respiratory Distress Syndrome, ARDS) Capillary endothelium and alveolar epithelium injury, and increased vascular permeability cause acute, progressive dyspnea, refractory hypoxemia, and pulmonary edema. Clinically, control of the primary disease and anti-infection treatment, mechanical ventilation support treatment, and drug (such as adrenocortical hormone) treatment are usually used to control the further development of the disease. [0003] Although drug therapy has achieved positive therapeutic effects in many patients with acute pneumonia, there are still a considerable number of patien...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/64A61K36/82A61P11/00
CPCA61K35/64A61K36/82A61P11/00A61K2236/331A61K2236/55A61K2236/51
Inventor 李强强吴黎明王凯薛晓锋
Owner BEE RES INST CHINESE ACAD OF AGRI SCI
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